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目的探讨女性无症状淋病患者中胸腺基质淋巴细胞生成素(TSLP)的表达及其与IL-17,IL-10水平的关系。方法选择123例女性无明显症状淋病患者为无明显症状组,32例有症状女性淋病患者为有症状组,放射免疫法(RIA)测定血清孕酮浓度;荧光定量PCR和酶联免疫吸附法(ELISA)在mRNA水平和蛋白水平检测宫颈分泌物中TSLP,IL-17和IL-10的水平。结果女性无明显症状淋病患者中TSLP表达升高,其表达与孕激素水平及IL-10呈显著正相关(r=0.99,0.97,P均<0.01),与IL-17呈显著负相关(r=-0.89,P<0.01)。有症状组和正常对照组TSLP和IL-10水平差异无统计学意义(P均>0.05)。有症状组IL-17水平明显高于正常对照组,差异有统计学意义(P<0.01)。结论孕激素可能通过上调TSLP表达在女性无症状淋球菌感染中发挥作用。
Objective To investigate the expression of thymic stromal lymphopoietin (TSLP) in female asymptomatic gonorrhea patients and its relationship with the levels of IL-17 and IL-10. Methods 123 cases of women with no obvious symptoms of gonorrhea were selected as symptom free group and 32 cases of symptomatic female gonorrhea as symptom group. Serum progesterone concentrations were determined by radioimmunoassay (RIA). The levels of serum progesterone were determined by fluorescence quantitative PCR and enzyme-linked immunosorbent assay ELISA) to detect levels of TSLP, IL-17 and IL-10 in cervical secretions at mRNA and protein levels. Results TSLP expression was significantly increased in women with no obvious symptoms of gonorrhea. The expression of TSLP was positively correlated with progesterone level and IL-10 (r = 0.99,0.97, P <0.01), but negatively correlated with IL-17 = -0.89, P <0.01). There was no significant difference in TSLP and IL-10 between the symptomatic group and normal control group (all P> 0.05). Symptomatic group IL-17 levels were significantly higher than the normal control group, the difference was statistically significant (P <0.01). Conclusion Progesterone may play a role in asymptomatic gonococcal infection in women by up-regulating TSLP expression.