论文部分内容阅读
目的观察慢性乙型肝炎病毒(hepatitis B virus,HBV)感染者血清中HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA)的分布特点及其与不同疾病状态的关系。方法2008年1月-12月收治慢性HBV感染者120例,男79例,女41例;年龄15~52岁,平均35岁。其中慢性HBV携带者21例,HBeAg阳性者38例,HBeAg阴性者35例,非活动性HBsAg携带者26例。采用巢式PCR法检测血清中HBV cccDNA。结果120例慢性HBV感染者血清中HBVcccDNA阳性总检出率为43.3%;慢性HBV携带者、HBeAg阳性者、HBeAg阴性和非活动性HBsAg携带者cccDNA阳性检出率分别为76.2%、64.7%、34.3%和0,各组间比较差异有统计学意义(P<0.05)。血清高HBV DNA定量组HBV cccDNA阳性检出率高于低HBV DNA定量组(P<0.05)。结论HBVcccDNA检出率与外周血HBV复制指标HBeAg、HBV DNA有显著的相关性,并与不同疾病状态相关。
Objective To investigate the distribution of HBV covalently closed circular DNA (cccDNA) in serum of patients with chronic hepatitis B virus (HBV) infection and its relationship with different disease states. Methods 120 cases of chronic HBV infection were admitted from January 2008 to December 2008, including 79 males and 41 females, aged from 15 to 52 years with an average of 35 years. Among them, 21 were chronic HBV carriers, 38 were HBeAg positive, 35 were HBeAg negative, and 26 were non-active HBsAg carriers. Nested PCR was used to detect serum HBV cccDNA. Results The total positive rate of HBVcccDNA in serum of 120 patients with chronic HBV infection was 43.3%. The positive rates of cccDNA in HBeAg-positive, HBeAg-negative and inactive HBsAg carriers were 76.2%, 64.7% respectively in chronic HBV carriers, 34.3% and 0, the difference between each group was statistically significant (P <0.05). The positive rate of HBV cccDNA in serum high HBV DNA quantitative group was higher than that of low HBV DNA quantitative group (P <0.05). Conclusion The detection rate of HBVcccDNA is significantly correlated with HBV replication markers HBeAg and HBV DNA in peripheral blood and is related to different disease states.