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目的了解河南省无偿献血人群HLA抗体的分布和特异性,评估无偿献血中因含有HLA抗体引起免疫性输血不良反应的风险。方法随机收集河南红十字血液中心已育女性血浆标本140(人)份、未育女性标本94(人)份,男性标本80(人)份,采用HLA抗体检测试剂盒对标本HLA抗体进行检测,阳性者进一步鉴定抗体特异性。结果 314名无偿献血者中,已育女性献血者HLA抗体检出阳性率27.86%(39/140),而未育女性献血者和男性献血者的阳性率仅为2.12%(2/94)和1.25%(1/80)。已育女性献血者中,HLA抗体出现频率较高的为抗A*23∶01、抗B*13∶01、抗B*13∶02、抗C*17∶01、抗DRB1*09∶01、抗DRB3*01∶01、抗DRB5*01∶01。结论已育女性献血者HLA抗体检出阳性率明显高于其他人群,为指导临床用血安全和制定相关政策提供了理论依据。
Objective To understand the distribution and specificity of HLA antibodies in blood donors in Henan Province and to evaluate the risk of immune-induced adverse transfusion reactions caused by HLA antibodies in blood donation. Methods A total of 140 (male) samples, 94 (male) samples from female fertility and 80 (male) samples from Henan Red Cross Blood Center were enrolled in this study. HLA antibodies were detected by HLA antibody detection kit, Positives further identified antibody specificity. Results The positive rate of HLA antibodies in 314 blood donors was 27.86% (39/140) in blood donors and 2.12% (2/94) in non-pregnant donors and male donors 1.25% (1/80). Among the female blood donors, HLA antibodies were more frequently found in anti-A * 23:01, anti B * 13:01, anti B * 13:02, anti C * 17:01, anti DRB1 * 09:01, Anti-DRB3 * 01:01, anti-DRB5 * 01:01. Conclusions The positive rate of HLA antibodies detected in pregnant female donors was significantly higher than that in other populations, providing a theoretical basis for guiding the clinical use of blood safety and formulating relevant policies.