论文部分内容阅读
目的:探讨阿托伐他汀对大鼠颈总动脉内膜损伤后血管平滑肌细胞离子泵活性和血管内膜增生的影响。方法:27只雄性SD大鼠随机分为假手术组(7只)、对照组和阿托伐他汀组(各10只)。后2组建立颈总动脉内膜损伤模型,阿托伐他汀组动物术前3d至术后28d每日接受阿托伐他汀(30mg·kg-1·d-1)治疗。术后28d处死大鼠,测定颈总动脉平滑肌细胞Na+K+ATP酶和Ca2+Mg2+ATP酶活性,应用苏木精伊红染色、计算机图像分析系统观察并测量动脉增生内膜厚度。结果:血管平滑肌细胞Na+K+ATP酶和Ca2+Mg2+ATP酶活性,阿托伐他汀组及假手术组均明显高于对照组(P<0.01)。对照组动脉最大增生内膜厚度明显高于阿托伐他汀组,P<0.01。结论:阿托伐他汀可能通过增高血管平滑肌细胞离子泵活性而抑制血管重塑的过程,从而在防治血管再狭窄中发挥作用。
Objective: To investigate the effect of atorvastatin on ion pump activity and intimal hyperplasia of vascular smooth muscle cells after carotid artery intimal injury in rats. Methods: Twenty-seven male Sprague-Dawley rats were randomly divided into sham operation group (n = 7), control group and atorvastatin group (n = 10). The carotid intima injury model was established in the latter two groups. Atorvastatin (30mg · kg-1 · d-1) was administered to the atorvastatin group three days before operation to 28 days after operation. After 28 days, the rats were sacrificed and the activities of Na + K + ATPase and Ca2 + Mg2 + ATPase of common carotid artery smooth muscle cells were measured. The thickness of arterial hyperplasia intima was observed and measured by computer image analysis system. Results: The activities of Na + K + ATPase and Ca2 + Mg2 + ATPase in vascular smooth muscle cells were significantly higher in atorvastatin group and sham operation group than those in control group (P <0.01). The thickness of the maximal hyperplastic intima in the control group was significantly higher than that in the atorvastatin group (P <0.01). Conclusion: Atorvastatin may play an important role in the prevention and treatment of vascular restenosis by increasing the activity of ion pump in vascular smooth muscle cells.