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介绍了以氢离子敏场效应管为基体电极，以碱性磷酸酶抗碱性磷酸酶抗体为例，在方法学上实现了电化学测量的一种酶免疫传感器．本生物传感器的响应时间达１８ｓ，标准曲线线性范围为７．８×１０（－２）μｇ／ｍｌ～０．６３μｇ／ｍｌ。另外，还讨论了适宜测量条件、抗体膜的寿命、抗原抗体复合物的寿命、抗原抗体复合物的分离及用不同抗体膜测量的重复性等。
An enzyme immuno-sensor based on hydrogen ion-sensitive field-effect transistor as substrate electrode and alkaline phosphatase anti-alkaline phosphatase antibody as an example was introduced. The biosensor response time of 18s, the standard curve of the linear range of 7.8 × 10 (-2) μg / ml ~ 0.63μg / ml. In addition, the suitable measurement conditions, the life span of the antibody membrane, the lifetime of the antigen-antibody complex, the separation of the antigen-antibody complex, the repeatability measured with different antibody membranes and the like are also discussed.