论文部分内容阅读
研究了不同剂量N+注入及低能N+介导大豆全DNA转入紫花苜蓿种子后,其发芽率、叶粗蛋白质含量及叶绿素含量等生理生化指标的变化规律。实验结果表明:在低剂量范围内(0～2.08×1016N+/cm2),N+注入对紫花苜蓿种子存在当代刺激效应。剂量在6.24×1016～8.32×1016N+/cm2范围内,N+注入对紫花苜蓿种子存在反常辐照损伤效应。随着N+注入剂量增加,各生理生化指标先降后升再降。紫花苜蓿种子经离子束注入刻蚀、加工处理,再用大豆基因组DNA浸泡后,M2总性状突变率达到19.8%,其中3株叶粗蛋白质含量比对照高约0.5%、1株叶绿素含量比对照高33.3%。
The changes of physiological and biochemical indexes such as germination rate, crude protein content and chlorophyll content of alfalfa seeds transformed with different doses of N + and low energy N + were studied. The experimental results show that there is a contemporary stimulating effect on the alfalfa seeds with N + implantation in the low dose range (0 ~ 2.08 × 1016N + / cm2). In the range of 6.24 × 10 16 ~ 8.32 × 10 16 N + / cm 2, the effect of N + injection on alfalfa seeds was abnormal. With the increase of N + injection dose, the physiological and biochemical indexes decreased first and then decreased again. The alfalfa seeds were etched and processed by ion beam implantation, and then soaked in soybean genomic DNA, the total mutation rate of M2 was 19.8%, of which three were about 0.5% higher than the control, and one chlorophyll content was higher than that of the control High 33.3%.