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In this paper, the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method. The result reveals that the adsorption of the protein-calmodulin contains two steps, i.e., one short quick step followed by a slow one. The complexation of calmodulin with Ca2+ was also first probed using EIS technique, in which the complexation of CaM with Ca2+ could be reflected by the change of apparent membrane capacitance(Capp) clearly. In all above measurements, a redox couple Fe(CN)63-/ Fe(CN)64- was used as probing-pin to reflect all the changes occurring in the above process. Our work suggests that some biological processes of CaM could be studied using EIS method conveniently.
In this paper, the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method. The result reveals the adsorption of the protein-calmodulin contains two steps, ie, one short quick step followed by The complexation of calmodulin with Ca2 + was also first probed using EIS technique, in which the complexation of CaM with Ca2 + could be reflected by the change of apparent membrane capacitance (Capp) clearly. In all above measurements, a redox couple Fe (CN) 63- / Fe (CN) 64- was used as probing-pin to reflect all the changes occurring in the above process. Our work suggests that those biological processes of CaM could be studied using EIS method conveniently.