论文部分内容阅读
本文用PAS反应及显示LDH和SDH(琥珀酸脱氢酶)活性等细胞化学方法研究了体外培养乳大鼠心肌细胞的形态发育以及细胞内糖原、LDH和SDH含量与搏动或停搏的关系.结果表明:①接种后,心肌细胞的形态必须发育到有突起的长柱状或星形,同时细胞内糖原、LDH及SDH含量均须达一定阈值(积分),细胞才能搏动.停搏细胞内,该三种物质的积分皆低于阈值。②心肌细胞密度越大,搏动力越强.培养的乳鼠心肌细胞可进行有丝分裂,增加细胞密度.培养48—72小时,部分细胞已经分裂,120小时分裂相最多。分裂时,糖原颗粒明显可见,细胞变短,但仍保留分枝状突起.细胞增殖成簇时,出现强有力的搏动. 上述细胞化学反应可用于细胞毒理学成药理学研究以评估细胞的受损程度.
In this study, we investigated the morphological development of cardiomyocytes cultured in vitro and the relationship between intracellular glycogen, LDH and SDH content and pulsation or arrest with PAS and cytochemical methods such as LDH and SDH (succinate dehydrogenase) The results showed that: ①After inoculation, the morphology of cardiomyocytes must develop into a long columnar or star with protrusions, and the intracellular glycogen, LDH and SDH content must reach a certain threshold (integral) The integrals of these three substances are below the threshold. The greater the density of cardiomyocytes, the stronger the beat force.The cultured neonatal rat cardiomyocytes can be mitotic and increase the cell density.Culture 48-72 hours, some cells have been split, 120 hours most of the split phase. When split, the glycogen granules are clearly visible, the cells become shorter, but still retain the branching protrusions, and powerful pulsations appear when the cells proliferate in clusters.The cytochemical reactions described above can be used in cytotoxicology into pharmacological studies to assess the effects of cells Loss level.