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【目的】筛选转基因鱼腥草,并初步分析外源基因在鱼腥草植株中的稳定性。【方法】以选择培养基中生长的转基因鱼腥草为材料,通过卡那霉素(Kan)叶片涂抹法进一步筛选抗性植株,并进行Kan抗性基因nptⅡ和目的基因CN(cecropin B/NP-1)的PCR分析。【结果】Kan叶片涂抹法筛选的最佳浓度为5 000 mg/L,观察时间以4 d为宜;经PCR检测验证,Kan抗性植株的nptⅡ基因阳性率达到96%,其中目的基因CN阳性率为37%。【结论】建立了适用于转基因鱼腥草的快速筛选方法,转基因鱼腥草中目的基因CN的稳定性低于抗性基因。
【Objective】 The purpose of this study was to screen the transgenic Houttuynia cordata Thunb., And to analyze the stability of foreign genes in Houttuynia cordata Thunb. 【Method】 The transgenic plants of Houttuynia cordata Thunb. Grown in selective medium were used as material to screen resistant plants by kanamycin (Kan) leaf smear method. The resistant gene npt Ⅱ and the target gene CN (cecropin B / NP -1) PCR analysis. 【Result】 The optimum concentration of Kan leaf smear was 5 000 mg / L and the observation time was 4 d. The positive rate of nptⅡ gene in Kan-resistant plants was 96% by PCR assay, and the positive gene of CN was positive The rate is 37%. 【Conclusion】 A rapid screening method for transgenic Houttuynia cordata was established. The stability of the target gene CN in transgenic Houttuynia was lower than that of the resistant gene.