论文部分内容阅读
目的 了解高三尖杉酯碱 (HHT)是否可通过激活Caspase 3诱导鼻咽癌细胞CNE 2Z凋亡。方法 将细胞分为 4组 :对照组、1mg/LHHT处理组 (HHT组 ) ,以及分别用Caspase抑制剂 (z VAD fmk)和Caspase 3抑制剂 (DEVD fmk)预处理 2h后 ,再用 1mg/LHHT处理的z VAD fmk +HHT组和DEVD fmk +HHT组。采用流式细胞术及荧光染色法 ,检测 4组细胞的凋亡率 ,以比色法检测它们中Caspase 3的相对活性。结果 流式细胞术和荧光染色均发现 ,HHT组的凋亡率明显高于其它各组 (P <0 .0 1) ;Caspase 3的活性于 2h开始升高 ,8h达高峰 ,明显高于其它各组 (P <0 .0 1) ,2 4h时同其它各组的差异无显著性 (P >0 .0 5 )。结论 HHT可通过激活Cas pase 3诱导CNE 2Z细胞凋亡 ,Caspase 3的活性升高具有时间依赖性
Objective To investigate whether homoharringtonine (HHT) can induce CNE 2Z apoptosis in nasopharyngeal carcinoma cells by activating Caspase 3. Methods The cells were divided into 4 groups: control group, 1mg / LHHT treatment group (HHT group), and pretreatment with Caspase inhibitor (z VAD fmk) and Caspase 3 inhibitor (DEVD fmk) LHV-treated z VAD fmk + HHT group and DEVD fmk + HHT group. Flow cytometry and fluorescence staining were used to detect the apoptosis rate of four groups of cells, and the relative activities of Caspase 3 in them were detected by colorimetric assay. Results The apoptosis rate of HHT group was significantly higher than that of other groups (P <0.01). The activity of Caspase 3 began to increase at 2h and reached its peak at 8h, which was significantly higher than that of other groups There was no significant difference between each group (P <0. 01) and other groups at 24 hours (P> 0.05). Conclusion HHT can induce apoptosis of CNE 2Z cells through activation of Caspase 3, and the activity of Caspase 3 increases in a time-dependent manner