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目的观察塑化剂邻苯二甲酸(2-乙基己基)酯(DEHP)对小鼠睾丸超微形态结构的影响,并研究蓝氧片(专利药用组合Y523)对小鼠睾丸生精细胞和支持细胞的保护和修复作用及其机制。方法 55只成年♂小鼠随机分为DEHP组、Y523组和对照组。DEHP组以大豆油为溶剂,剂量分别为:0,10,20,50,和100 mg·kg-1·d-1,连续30 d灌胃染毒;Y523组以等量大豆油混合1 000 mg蓝氧片原料粉剂连续30 d灌胃给药后重复DEHP组的灌胃染毒过程;对照组以标准饲料同期喂养。采用透射电镜观察小鼠睾丸超微结构的改变。结果与对照组相比,随着DEHP灌胃染毒剂量的递增,DEHP组小鼠睾丸生精细胞出现胞膜塌陷,核质疏松,小细胞器消失,空泡形成,甚至整个生精细胞层消失;支持细胞胞膜皱缩,核质浓缩,胞质内空泡形成和凋亡小体样结构。与DEHP组相比,蓝氧片组小鼠睾丸生精细胞和支持细胞的超微形态结构改变与对应剂量DEHP灌胃染毒的DEHP组小鼠基本类似,但胞质内线粒体、溶酶体等小细胞器的数量明显增多。结论 DEHP灌胃染毒可致小鼠睾丸生精细胞空泡样变性退化,细胞胀亡和凋亡。蓝氧片对DEHP所致的睾丸损害有一定的保护作用,其机制可能与小鼠睾丸支持细胞内线粒体和溶酶体等细胞器数量的增加有关。
Objective To observe the effect of plasticizer phthalate (DEHP) on the ultrastructure of mouse testis and to study the effect of blue-oxygen tablet (patented drug combination Y523) on the testis spermatogenic cells And support the protection and repair of cells and their mechanisms. Methods 55 adult ♂ mice were randomly divided into DEHP group, Y523 group and control group. In the DEHP group, soybean oil was used as the solvent, and the doses were 0, 10, 20, 50, and 100 mg · kg-1 · d-1 respectively for 30 days. mg of blue-chip raw powder for 30 days after oral administration of repeated administration of DEHP group intragastric administration; the control group fed with standard feed over the same period. Transmission electron microscopy was used to observe the ultrastructural changes of testis in mice. Results Compared with the control group, the DEHP group mice showed germ cell collapse, loose nucleolus, disappearance of small organelles, vacuolization and disappearance of the entire spermatogenic cell layer ; Supporting cell membrane shrinkage, nuclear condensation, cytoplasmic vacuoles and apoptotic body-like structures. Compared with the DEHP group, the ultrastructural changes of spermatogenic cells and supporting cells in the testis of the blue tablet group were similar to those of the DEHP group treated with DEHP. However, the contents of mitochondria, lysosomes The number of small organelles increased significantly. Conclusion DEHP intragastric administration induced degeneration of vacuolar degeneration of spermatogenic cells, cell expansion and apoptosis in mouse testes. Oxygen can protect the testes induced by DEHP to a certain extent, and its mechanism may be related to the increase of the number of organelles such as mitochondria and lysosome in supporting cells of mice testis.