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目的：研究肠缺血再灌注过程对局部及远隔器官中性粒细胞（ＰＭＮ）和组织血管内皮细胞粘附分子表达的影响及其与脏器功能受损的关系。方法：雄性Ｗｉｓｔａｒ大白鼠，随机分为对照组、肠缺血组和再灌注组。比较肠缺血再灌注过程中局部及远隔器官组织髓过氧化物酶（ＭＰＯ）活性、ＰＭＮ上的配基ＣＤ１１ｂ／ＣＤ１８表达、细胞间粘附分子１（ＩＣＡＭ１）表达的变化以及主要脏器功能的改变。结果：①主要脏器功能变化异常的峰值均在再灌注后１小时～２小时，与组织ＭＰＯ活性升高的峰值时相基本一致；②再灌注１小时和２小时血清可刺激ＰＭＮ上ＣＤ１１ｂ／ＣＤ１８表达上调，与肺组织ＭＰＯ活性呈正相关关系；③肠缺血期，肠局部ＩＣＡＭ１ｍＲＮＡ表达有所增加，肠缺血及再灌注早期蛋白水平表达增加；肝及肺组织ＩＣＡＭ１ｍＲＮＡ表达有所增加，肠缺血及再灌注早期蛋白水平表达增加；肝及肺组织ＩＣＡＭ１ｍＲＮＡ表达及血管内皮细胞ＩＣＡＭ１分布在再灌注后的２小时和６小时增加。结论：缺血再灌注后肠血管可能作为“预激床”激活循环中ＰＭＮ，从而构成再灌注后ＰＭＮ在远隔器官聚集、活化，造成组织损伤的病理生理学基础；ＰＭＮ上ＣＤ１１ｂ／ＣＤ１８及血管内皮细胞上ＩＣＡＭ１表达上调则构?
Objective: To investigate the effect of intestinal ischemia / reperfusion on the expression of adhesion molecules in neutrophils (PMN) and vascular endothelial cells (VEGF) in local and remote organs and its relationship with impaired organ function. Methods: Male Wistar rats were randomly divided into control group, intestinal ischemia group and reperfusion group. To compare the changes of MPO activity, ligand CD11b / CD18 expression and ICAM1 expression in the local and remote organs during intestinal ischemia-reperfusion, Functional changes. RESULTS: ① The peak of abnormal function of major organs were all within 1 hour ~ 2 hours after reperfusion, which was consistent with the peak phase of MPO activity. ② The reperfusion of 1 hour and 2 hours of reperfusion could stimulate the expression of CD11b / CD18 expression was positively correlated with MPO activity in lung tissue. ③In intestinal ischemia, the expression of ICAM1 mRNA in intestine was increased, the expression of ICAM1 mRNA in intestine ischemia and reperfusion increased, the expression of ICAM1 mRNA in liver and lung increased, The expression of ICAM1mRNA in liver and lung tissue and the distribution of ICAM1 in vascular endothelial cells increased at 2h and 6h after reperfusion. CONCLUSIONS: After ischemia-reperfusion, the intestinal vessels may activate PMN as a “pre-stimulant bed”, thus forming a pathophysiological basis for the PMN to accumulate and activate in distant organs after reperfusion, resulting in tissue injury. CD11b / CD18 and vascular ICAM1 expression on endothelial cells is the structure?