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目的检测Th1/Th2型细胞因子在实验性矽肺大鼠肺组织中的动态表达水平。方法将SPF级成年雄性SD大鼠随机分为染尘组和对照组,每组42只。模型组采用非气管暴露法一次性注入1 m L二氧化硅悬液(100 mg/m L)建立大鼠矽肺模型,对照组气管内注入等量灭菌生理盐水。分别在染尘第1天、1周、2周、3周、6周、9周和12周各处死6只大鼠,采用酶联免疫吸附实验(ELISA)检测大鼠肺组织匀浆中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白介素18(IL-18)、白介素4(IL-4)、白细胞介素10(IL-10)及IFN-γ/IL-4的表达水平。结果染尘组TNF-α表达量均高于对照组(P<0.05);染尘组IFN-γ在染尘第1天~2周表达量高于对照组(P<0.05);染尘组IL-18表达量均高于对照组,差异无统计学意义;染尘组IL-4在染尘第1周表达量低于对照组(P<0.05),第9周及12周高于对照组(P<0.05);染尘组IL-10在染尘第6~9周表达量高于对照组(P<0.05);染尘组Th1/Th2比值随染尘时间增长而降低,染尘组Th1/Th2在染尘第1天~2周比值高于对照组(P<0.05)。结论在矽肺的发生发展过程中细胞因子发生变化:Th1型细胞因子持续高表达,Th2型细胞因子表达量先降低后升高。
Objective To detect the dynamic expression of Th1 / Th2 cytokines in the lung of experimental silicosis rats. Methods SPF adult male Sprague-Dawley rats were randomly divided into dust-exposed group and control group, 42 rats in each group. In the model group, the model of silicosis was established by injecting 1 mL suspension of silicon dioxide (100 mg / mL) into the rat model by non-tracheal exposure. The control group was injected with sterile normal saline. Six rats were sacrificed on day 1, week 1, week 2, week 3, week 6, week 9, and week 12 respectively. Tumors in the lung homogenates were detected by enzyme-linked immunosorbent assay (ELISA) TNF-α, IFN-γ, IL-18, IL-4, IL-10 and IFN-γ / IL-4 expression levels. Results The expression of TNF-α in the dyed group was higher than that in the control group (P <0.05). The expression of IFN-γ in the dyed group was higher than that in the control group (P <0.05) The expression of IL-18 in the dust-exposed group was lower than that in the control group (P <0.05), and higher than that of the control group at 9 and 12 weeks (P <0.05). The expression of IL-10 in the dust-exposed group was higher than that in the control group (P <0.05) at 6 to 9 weeks after exposure to dust. The Th1 / Th2 ratio in the dust- The ratio of Th1 / Th2 in the first day to the second week of dusting was higher than that in the control group (P <0.05). Conclusion The changes of cytokines during the development of silicosis: Th1-type cytokines continued to be highly expressed, and the expression of Th2-type cytokines decreased first and then increased.