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目的探讨不同饮食限制(dietary restriction,DR)水平对小鼠学习记忆、抗氧化能力影响。方法120只ICR小鼠随机分为自由饮食(对照)组,20%,40%,60%DR组,连续25 d,进行力竭游泳及耐缺氧实验;39 d后,进行Y迷宫、水迷宫、脏器系数、超氧化物歧化酶(SOD)及丙二醛(MDA)含量的测定。结果Y迷宫结果表明,40%DR组记忆成绩(14.40±2.68)次,与自由饮食组比较差异有统计学意义(P<0.05);水迷宫结果表明,40%DR组潜伏期(20.36±9.12)s,访问次数(1.5±0.5)次,游动距离(0.49±0.32)m。;60%DR组脑系数为(1.51±0.19)%,明显高于自由饮食组(1.12±0.09)%(P<0.01);各饮食限制组小鼠脾脏、甲状腺系数及肾脏丙二醛(MDA)含量均有所下降;小鼠耐力及耐缺氧能力与限制水平成正比,雌鼠耐缺氧能力强于雄鼠。结论适当饮食限制可以提高机体对新环境适应能力,提高体内抗氧化系统活性。
Objective To investigate the effects of different dietary restriction (DR) levels on learning and memory and antioxidant capacity in mice. Methods 120 ICR mice were randomly divided into two groups: free diet (control group), 20%, 40%, 60% DR group for continuous 25 days. The rats were subjected to exhaustive swimming and hypoxia tolerance test. Maze, organ coefficient, superoxide dismutase (SOD) and malondialdehyde (MDA) content were determined. Results The results of Y maze test showed that the memory score of the 40% DR group was (14.40 ± 2.68) times, compared with that of the free diet group (P <0.05). The water maze results showed that the latent period of the 40% DR group was (20.36 ± 9.12) s, the number of visits (1.5 ± 0.5) and the distance of swimming (0.49 ± 0.32) m. (1.51 ± 0.19)% in the 60% DR group were significantly higher than those in the free diet group (1.12 ± 0.09%) (P <0.01). The spleen and thyroid gland numbers of the mice in each diet restriction group and the malondialdehyde (MDA) ) Content decreased; mice endurance and hypoxia tolerance is proportional to the level of restriction, female hypoxia tolerance than male rats. Conclusion Appropriate dietary restriction can improve the ability of the body to adapt to the new environment and improve the anti-oxidative system activity in vivo.