论文部分内容阅读
目的:研究死亡结构域相关蛋白(Daxx)基因在小鼠睾丸精子发生过程中的表达特征,初步探讨其在生精过程中的作用。方法:通过实时荧光定量PCR(q PCR)、Western印迹及免疫荧光等方法检测Daxx在不同周龄野生型小鼠睾丸组织以及成年睾丸支持细胞雄激素受体特异性敲除(SCARKO)和雄激素受体敲除(ARKO)小鼠睾丸中的表达特征。结果:q PCR、Western印迹和免疫荧光结果表明,Daxx基因在出生4周后小鼠睾丸中高表达,且主要定位于细胞核;与野生型小鼠相比,SCARKO小鼠睾丸中DAXX的表达差异不显著(0.853±0.058 vs1.000±0.015),但在生精细胞细胞核中呈极性分布;DAXX在ARKO小鼠睾丸表达显著降低(0.299±0.026 vs1.000±0.015,P<0.01)。结论 :Daxx基因在小鼠睾丸发育中期时表达最高。ARKO小鼠中DAXX的表达与野生型相比显著降低,睾丸支持细胞中AR基因特异性敲除影响DAXX定位。DAXX可能参与调控小鼠的精子发生过程。
OBJECTIVE: To study the expression of Daxx gene during testicular spermatogenesis in mice and to explore its role in spermatogenesis. Methods: The expression of androgen receptor specific knockout (SCARKO) and androgen receptor in testis tissues of adult mice and adult testis supporting cells of Daxx in different weeks of age were detected by real-time quantitative PCR (qPCR), Western blotting and immunofluorescence The expression characteristics of the body testosterone (ARKO) mouse testis. Results: qPCR, Western blotting and immunofluorescence results showed that Daxx gene was highly expressed in the testes of mice 4 weeks after birth and mainly located in the nucleus. Compared with wild-type mice, the expression of DAXX in testis of SCARKO mice was not significantly different (0.853 ± 0.058 vs1.000 ± 0.015), but it was polar distribution in the nucleus of germ cell. The expression of DAXX in testis of ARKO mice was significantly decreased (0.299 ± 0.026 vs1.000 ± 0.015, P <0.01). Conclusion: Daxx gene is most expressed in the mid-testis of mice. DAXX expression was significantly reduced in ARKO mice compared with wild type, and AR gene-specific knockout in testicular support cells affected DAXX localization. DAXX may be involved in the regulation of mouse spermatogenesis.