【摘 要】
：
血清中和抗体滴度的大小反映血清对病毒感染的中和能力,其是评价疫苗免疫效果和病毒感染状况的重要指标,而液相阻断酶联免疫吸附试验(liquid phase block ELISA,LPB-ELISA)是
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血清中和抗体滴度的大小反映血清对病毒感染的中和能力,其是评价疫苗免疫效果和病毒感染状况的重要指标,而液相阻断酶联免疫吸附试验(liquid phase block ELISA,LPB-ELISA)是一种通过ELISA检测抗体滴度的方法,因此研究LPB-ELISA抗体滴度和中和抗体滴度的关系对疫苗评价和理论研究有重要意义.本研究采用野外采集的90份牛血清进行了中和试验和LPB-ELISA试验,结果表明中和抗体滴度和LPB-ELISA抗体滴度具有相关性,相关系数为0.642,但是试验证明有41%中和抗体阴性样品会被LPB-ELISA检测为阳性,通过改变LPB-ELISA的阳性判断标准,采用抑制率>0.7的抗体滴度计算方法和1/8判定标准,可以使改进的LPB-ELISA的计算结果和中和试验结果更好的符合,降低假阳性率,使LPB-ELISA更适合进行口蹄疫血清学监测和普查.
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