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植物名称:金苞花(Pachystachys lutea Nees),为爵床科,厚穗爵床属植物。 材料类别:嫩茎,常规消毒后切成有一对腋芽的茎段。 培养条件:以MS为基本培养基,诱导丛生芽分化和继代繁殖时附加6—BA3毫克/升,NAA0.1毫克/升,0.76%腐殖酸钠100毫克/升。生根培养基为MS_0。诱导丛生芽分化每天光照12小时,光照度2000——3000lx。继代繁殖与诱导生根采用自然散射光照射,每天光照约13——15小时,光照度2000——6000lx。培养温度20——28℃。 生长与分化情况:在MS附加6—BA3毫克/升,NAA0.1毫克/升的培养基上,接种第三天外植体切面开始产生白色愈伤组织,第六天腋芽成对萌发并迅速生长,一月
Botanical Name: Pachystachys lutea Nees, is a king bed family, thick Superstar bed bed plant. Material category: tender stems, cut into a pair of axillary buds after routine disinfection. Culture conditions: MS as a basic medium to induce clustered bud differentiation and subculture of additional 6-BA3 mg / L, NAA0.1 mg / L, 0.76% sodium humate 100 mg / L. Rooting medium MS_0. Induction of clustered bud differentiation daily light 12 hours, illumination 2000 - 3000lx. Successive generations of breeding and induced rooting using natural light scattering, light every day for about 13 - 15 hours, light intensity 2000 - 6000lx. Culture temperature 20-28 ℃. Growth and differentiation: In the MS supplemented with 6-BA3 mg / L, NAA0.1 mg / L medium, white explants began to produce white callus on the third day after inoculation. On the sixth day, the axillary buds germinated and grew rapidly ,January