论文部分内容阅读
目的 研究β-七叶皂甙(β-aescin)对大鼠急性脑损伤后脑组织核因子-κB(NF-κB)活性和肿瘤坏死因子-α(TNF-α)蛋白表达的影响。 方法 62只SD大鼠采用自由落体致伤法制作脑外伤模型,随机分成四组: (1)假手术对照组(A); (2)创伤组(B); (3)β-七叶皂甙治疗组(C); (4)吡咯烷二硫基甲酸酯(PDTC)治疗组(D)。每组分别在术后6, 24h和3d三个时相点收取脑组织标本,采用凝胶电泳迁移率分析(EMSA)测定脑组织NF-κB活性;放射免疫法测定脑组织TNF-α蛋白水平,并测定脑组织含水量及进行病理形态学观察。 结果 与假手术对照组比较,大鼠脑损伤后脑组织NF-κB活性和TNF-α蛋白水平以及脑组织含水量显著增高(P<0. 01);与创伤组比较,β-七叶皂甙和PDTC治疗组脑组织NF-κB活性(P<0. 01 )、脑组织TNF-α蛋白水平(P<0. 01)及脑组织含水量(P<0. 05)均显著降低。 结论 β-七叶皂甙可以抑制NF-κB活化,下调TNF-α蛋白表达,从而减轻脑水肿。这可能是β-七叶皂甙治疗创伤性脑水肿的分子生物学作用机制之一。
Objective To study the effect of β-aescin on the activity of nuclear factor-κB (NF-κB) and the expression of tumor necrosis factor-α (TNF-α) protein in rat brain after acute brain injury. Methods Sixty-two Sprague-Dawley rats were subjected to free-fall injuries to make brain injury models and randomly divided into four groups: (1) sham-operated control group (A); (2) trauma group (B); (3) β-aescin Treatment group (C); (4) Pyrrolidine dithiocarbamate (PDTC) treatment group (D). The brain tissue samples were collected at the three time points of 6, 24 and 3 days after operation. The activity of NF-κB in brain tissue was determined by electrophoretic mobility shift assay (EMSA). The level of TNF-α protein in brain tissue was measured by radioimmunoassay. , And determine the brain tissue water content and pathological observation. Results Compared with the sham-operated control group, the activity of NF-κB and TNF-α protein in brain tissue and the water content of brain tissue were significantly increased after brain injury in rats (P<0.01); β-aescinate was compared with the trauma group. PDTC treatment group brain tissue NF-κB activity (P <0. 01), brain tissue TNF-α protein levels (P <0. 01) and brain tissue water content (P <0. 05) were significantly reduced. Conclusion β-Aescin can inhibit the activation of NF-κB, down-regulate the expression of TNF-α protein and reduce cerebral edema. This may be one of the molecular biological mechanisms of β-aescin in the treatment of traumatic brain edema.