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采用不同剂量60Co-γ射线辐照降解壳聚糖,研究了其降解产物对玉米生长的调节作用。研究发现,壳聚糖辐照产物可提高玉米种子发芽率,促进玉米幼苗生长,提高玉米抗逆相关生理生化指标。辐照剂量为200kGy,壳聚糖辐照产物溶液浓度为3.0mg/mL时,可显著提高玉米种子萌发率和促进芽长;浓度为4.0mg/mL时,显著促进主根长度;浓度为2.0mg/mL~3.0mg/mL,苯丙氨酸解氨酶(PAL)活性显著高于对照。当辐照剂量为200kGy~400kGy,产物溶液浓度为2.0mg/mL~4.0mg/mL时,能显著提高胚乳中α-淀粉酶活性和幼苗中叶绿素含量;浓度为2.0mg/mL~3.0mg/mL时,显著提高幼苗过氧化物酶(POD)的活性。综合多种影响因素得出:利用壳聚糖辐照产物开发玉米促生抗逆剂的适宜60Co-γ射线辐照剂量为200kGy~300kGy,浓度为2.0mg/mL~3.0mg/mL。
The chitosan was degraded by different doses of 60Co-γ ray and the regulation of its degradation products on the growth of maize was studied. The results showed that irradiation with chitosan could increase the germination rate of maize seeds, promote the growth of maize seedlings and enhance the physiological and biochemical indexes related to maize stress resistance. When the irradiation dose was 200kGy and the concentration of chitosan irradiation solution was 3.0mg / mL, the germination rate and bud length of maize seeds were significantly increased. When the concentration was 4.0mg / mL, the length of main roots was significantly promoted. The concentration of 2.0mg /mL ~ 3.0mg/mL, phenylalanine ammonia lyase (PAL) activity was significantly higher than the control. When the irradiation dose was 200kGy ~ 400kGy and the concentration of the product solution was 2.0mg / mL ~ 4.0mg / mL, the α-amylase activity in endosperm and the content of chlorophyll in seedling were significantly increased. The concentration of chlorophyll was 2.0mg / mL ~ 3.0mg / mL significantly increased peroxidase (POD) activity in seedlings. Based on a variety of influencing factors, the optimum dosage of 60Co-γ ray irradiation for developing corn anti-infestation agent was 200kGy ~ 300kGy with concentration of 2.0mg / mL ~ 3.0mg / mL.