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羊水腺苷脱氨酶(ADA)活性的测定有助于产前检查重症联合免疫缺陷症,但羊水中ADA活性偏低,难于直接检出。作者报道以氨气敏电极,应用标准加入法测定羊水中ADA活性。此法简便、快速,8 个不同样本测定的回收率为100.89±1.80％;7次测定的变异系数为1.64％。对62例13～41周正常妊娠羊水测定,ADA活性均值为0.267±0.173u(M±SD)。
Determination of amniotic adenosine deaminase (ADA) activity in prenatal diagnosis of severe combined immunodeficiency, but amniotic fluid ADA activity is low, it is difficult to detect directly. The authors report using ammonia gas sensing electrode, ADA activity in amniotic fluid using standard addition method. The method is simple and rapid. The recovery of eight different samples was 100.89 ± 1.80%. The coefficient of variation of seven determinations was 1.64%. The mean ADA activity of 62 cases of normal pregnancy amniotic fluid from 13 to 41 weeks was 0.267 ± 0.173u (M ± SD).