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建立了蔬菜中三嗪类农药(triazine)(包含莠灭净、莠去津、扑灭通、扑草净、扑灭津、西玛津、特丁净7种化合物)的高效液相色谱串联三重四极杆线性离子阱复合质谱(HPLC-QTrap)的检测方法。方法参考国标对蔬菜进行提取,净化处理后采用高效液相色谱分离,电喷雾三重四极杆线性离子阱复合质谱检测。对样品的阳性色谱峰进行鉴定,包括谱库的建立以及搜库比对等方面研究,并且可以同时根据方法的校正曲线进行定量。结果表明,7种三嗪类农药在0.1～50μg/L范围内定量离子的峰面积和样品的质量浓度之间有很好的线性关系;利用QTrap质谱的优势,在化合物质量浓度大于1μg/L时,可得到高质量的二级子离子全扫描数据,可以有效的对色谱峰进行鉴定,并且方法的加标回收率在85%～101%之间,相对标准偏差小于8%。该方法简便、灵敏、准确,可用于蔬菜中多种农药的定性定量同时检测。
A high performance liquid chromatography (tandem) triple quadruplex (triazine) containing 7 compounds of ametrine, atrazine, trimebutine, prometryn, fenvastatin, Determination of linear-ion trap mass spectrometry (HPLC-QTrap). Methods The vegetables were extracted with reference to the national standard and purified by high performance liquid chromatography and electrospray triple quadrupole linear ion trap mass spectrometry. The positive peak of the sample was identified, including the establishment of the library and the comparison of the library search and other aspects, and can be based on the method of calibration curve for quantitative. The results showed that there was a good linear relationship between the peak area of the quantified ions and the mass concentration of the sample in the range of 0.1 ~ 50μg / L for seven triazine pesticides. Using the advantage of QTrap mass spectrometry, when the mass concentration of the compound was more than 1μg / L , High-quality secondary ion full scan data can be obtained, which can effectively identify the chromatographic peaks. The recoveries of the method are between 85% and 101%, and the relative standard deviations are less than 8%. The method is simple, sensitive and accurate and can be used for the simultaneous qualitative and quantitative detection of multiple pesticides in vegetables.