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目的:研究盐酸异丙嗪(promethazine hydrochloride)在DNA修饰碳糊电极(DNA/CPE)上的电化学行为及电化学动力学性质。方法:用循环伏安法(CV)、线性扫描伏安法(LSV)、计时库仑法(CC)和计时电流法(CA)等多种电化学方法进行研究。结果:在pH=6.8的C-L缓冲溶液中,1.0×10-4mol.L-1的盐酸异丙嗪在DNA/CPE上氧化峰电位为0.633 V,比在碳糊电极(carbon paste electrode,CPE)上的峰电位正移了30 mV,峰电流显著降低。测得盐酸异丙嗪在DNA/CPE上电极反应动力学参数:电荷转移系数α=0.80,扩散系数D=8.936×10-6cm2.s-1,电极反应速率常数kf=3.828×10-2cm2.s-1,而在CPE上α=0.86,扩散系数D=1.099×10-5cm2.s-1。结论:盐酸异丙嗪与DNA二者以嵌插作用结合,形成了一种非电活性物质。
Objective: To study the electrochemical behavior and electrochemical kinetics of promethazine hydrochloride on DNA modified carbon paste electrode (DNA / CPE). Methods: Various electrochemical methods such as cyclic voltammetry (CV), linear sweep voltammetry (LSV), chrono-coulometry (CC) and chronoamperometry (CA) Results: In the CL buffer solution of pH = 6.8, 1.0 × 10-4mol·L-1 Promethazine had a peak oxidation potential of 0.633 V on DNA / CPE, The peak potential is positive shifted 30 mV, peak current decreased significantly. Measured promethazine hydrochloride in the DNA / CPE electrode reaction kinetics parameters: charge transfer coefficient α = 0.80, the diffusion coefficient D = 8.936 × 10-6cm2.s-1, the electrode reaction rate constant kf = 3.828 × 10-2cm2. s-1 and α = 0.86 on the CPE with a diffusion coefficient D = 1.099 × 10 -5 cm 2 .s -1. CONCLUSION: Promethazine hydrochloride and DNA are both intercalated and form a non-electroactive substance.