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目的采用RP-HPLC法测定景天三七不同产地和药用部位中没食子酸的含量。方法采用Kromasil C18色谱柱(250mm×4.6 mm,5μm),流动相为甲醇-5%醋酸溶液(5:95),流速1 ml.min-1,柱温30℃,检测波长274 nm。结果没食子酸0.2026~1.2056μg与峰面积的线性关系良好;回归方程为:Y=3.627×106X+7.089×105(r=0.9998,n=6);平均回收率为99.5%,RSD=0.73%(n=9)。结论所建方法简便、准确、重复性好,可用于控制民族药景天三七药材的质量。
Objective To determine the content of gallic acid in different producing areas and medicinal parts of Sanqi, S. chinensis by RP-HPLC. Methods A Kromasil C18 column (250mm×4.6mm, 5μm) was used. The mobile phase was methanol-5% acetic acid solution (5:95), the flow rate was 1 ml.min-1, the column temperature was 30°C, and the detection wavelength was 274 nm. Results The linear relationship between the range of 0.2026-1.2056μg of gallic acid and the peak area was good; the regression equation was: Y=3.627×106X+7.089×105 (r=0.9998, n=6); the average recovery was 99.5%, RSD was 0.73% ( n=9). Conclusion The established method is simple, accurate, and reproducible. It can be used to control the quality of the medicinal drug King Sedum notoginseng.