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Objective Ginsenoside Rg3was prepared by converting protopanaxadiol(PPD)-type saponins using lemon juice as the catalyst.Methods Preparation of ginsenoside Rg3 was optimized by response surface method(RSM)based on a four-factor and five-level central composite design.Results The optimal yield of ginsenoside Rg3was predicted to be 75.98%in the combination of the factors(PPD-type saponins concentration of 23.6 mg/mL,lemon juice concentration of 97.6%at 85.7℃for 130.0 min)through the canonical analysis and ridge analysis with maximum responses.Under the optimum conditions,the actual yield of ginsenoside Rg3was 75.57%.Conclusion RSM is effective to optimize the preparation of ginsenoside Rg3by lemon-catalyzed PPD-type saponins.The achievement for the preparation of ginsenoside Rg3would promote the development of health-care production.
Objective Ginsenoside Rg3was prepared by converting protopanaxadiol (PPD) -type saponins using lemon juice as the catalyst. Methods Preparation of ginsenoside Rg3 was optimized by response surface method (RSM) based on a four-factor and five-level central composite design. Results The optimal yield of ginsenoside Rg3was predicted to be 75.98% in the combination of the factors (PPD-type saponin concentration of 23.6 mg / mL, lemon juice concentration of 97.6% at 85.7 ° C for 130.0 min) through the canonical analysis and ridge analysis with maximum responses.Under the optimum conditions, the actual yield of ginsenoside Rg3was 75.57%. Confrc RSM is effective to optimize the preparation of ginsenoside Rg3by lemon-catalyzed PPD-type saponins. The achievement for the preparation of ginsenoside Rg3would promote the development of health-care production.