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目的检测宫颈正常组织以及不同临床分期宫颈癌组织中转移抑制基因1(metastasis suppressor 1,MTSS1)的表达,分析该基因在各组织中的变化与临床病理因素的关系,并初步探讨该基因在宫颈癌发生发展及转移过程中的作用及分子机制。方法取2011年12月至2014年12月期间采集的103例宫颈组织,病理切片诊断患者宫颈组织类型,判断分化程度,应用实时荧光定量PCR(q-PCR)和Western印迹法检测不同宫颈组织中MTSS1基因及其蛋白的表达水平。结果 q-PCR结果提示,ⅡB-Ⅳ期宫颈癌组MTSS1基因表达量明显高于正常宫颈组及Ⅰ-ⅡA期宫颈癌组,3组间均有明显差异(P=0.000);Western印迹检测MTSS1蛋白在正常宫颈组织中的阳性表达率为23.3%,在宫颈癌组织中为53.3%,两组间差异明显(P=0.002);MTSS1在宫颈癌组织中的表达与年龄、分化以及有无淋巴结转移均无明显相关性(P>0.05),而在临床分期中,ⅡB-Ⅳ期MTSS1蛋白表达率明显高于Ⅰ-ⅡA期的宫颈癌组织(P=0.005)。结论 MTSS1的表达与宫颈癌的临床分期呈正相关趋势,该基因可能在宫颈癌的发生发展中起着重要的作用。
Objective To detect the expression of metastasis suppressor 1 (MTSS1) in normal tissues and cervical cancer tissues with different clinical stages, analyze the relationship between the changes of this gene and clinicopathological factors, The role and molecular mechanism of carcinogenesis and metastasis. Methods 103 cases of cervical tissue collected from December 2011 to December 2014 were collected. Pathological examination was used to diagnose the type of cervical tissue and determine the degree of differentiation. Real-time quantitative PCR (q-PCR) and Western blotting were used to detect the changes of cervical tissue in different cervical tissues MTSS1 gene and its protein expression levels. Results The q-PCR results showed that the expression of MTSS1 gene in stage ⅡB-Ⅳ cervical cancer was significantly higher than that in normal cervical cancer group and stage Ⅰ-ⅡA cervical cancer group (P = 0.000); Western blotting detected MTSS1 The positive expression rate of protein in normal cervical tissue was 23.3% and in cervical cancer tissue was 53.3%, the difference was significant between the two groups (P = 0.002). The expression of MTSS1 in cervical cancer tissues was related to age, differentiation and lymph node (P> 0.05). However, in clinical stage, the expression of MTSS1 in stage ⅡB-Ⅳ was significantly higher than that in stage Ⅰ-ⅡA cervical cancer (P = 0.005). Conclusion The expression of MTSS1 positively correlates with the clinical stage of cervical cancer. The gene may play an important role in the development of cervical cancer.