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目的探讨降铅Ⅰ号对低水平铅暴露仔鼠脑细胞凋亡的干预作用及神经行为改善的机制。方法成年Wistar大鼠按雌∶雄=2∶1比例交配后,随机分为正常组和实验组。从孕期第1天起,分别饮用蒸馏水和0.2%醋酸铅溶液,直至第21天仔鼠断乳,断乳仔鼠用于实验。实验组仔鼠随机分为铅模型组、EDTA对照组、降铅Ⅰ号低剂量组、降铅Ⅰ号中剂量组、降铅Ⅰ号高剂量组。正常组仔鼠作为空白组。各组给予相应干预。采用原子吸收分光光度石墨炉法测定血铅和脑铅含量,用Y迷宫观察用药后对仔鼠学习和记忆能力的影响。结果治疗后各治疗组仔鼠的血铅水平均显著低于铅模型组(P<0.001);EDTA组、降铅Ⅰ号低剂量组的脑铅水平与铅模型组比较明显下降(P<0.05),降铅Ⅰ号各组的脑铅水平与EDTA组比较无差异(P>0.05);降铅Ⅰ号低剂量组的记忆保持率与空白组比较差异无统计学意义(P>0.05)。降铅Ⅰ号低剂量组、降铅Ⅰ号高剂量组的记忆保持率高于铅模型组(P<0.05,P<0.001)。降铅Ⅰ号低剂量组的记忆保持率高于EDTA组(P<0.01),降铅Ⅰ号低剂量组的记忆保持率高于降铅Ⅰ号中剂量组(P<0.05);各治疗组较铅模型组海马凋亡标记指数均显著下降,差异显著(P<0.05,P<0.01)。结论降铅Ⅰ号能降低低水平铅暴露仔鼠的血铅和脑铅含量,明显改善低水平铅暴露仔鼠的学习记忆能力。
Objective To investigate the effects of Jiangzao I on the apoptosis of brain cells of offspring exposed to low levels of lead and the mechanism of neurobehavioral improvement. Methods Adult Wistar rats were mated with male and female at a 2: 1 ratio and then randomly divided into normal group and experimental group. From the first day of pregnancy, respectively, drinking distilled water and 0.2% lead acetate solution until the 21st day offspring offspring, offspring offspring used in the experiment. The experimental group of rats were randomly divided into lead model group, EDTA control group, low-dose lead group Ⅰ, low-dose lead group Ⅰ, high-dose lead group Ⅰ. Normal pups as a blank group. Each group was given the corresponding intervention. The blood lead and lead levels were determined by atomic absorption spectrophotometric graphite furnace method. The effects of drug administration on the learning and memory ability of offspring were observed with Y maze. Results After treatment, the level of blood lead in the offspring of each treatment group was significantly lower than that in the lead model group (P <0.001). The levels of lead in the EDTA group and the lead-1 low-dose group were significantly lower than those in the lead model group (P <0.05 (P> 0.05). There was no significant difference in the level of memory between the two groups (P> 0.05). There was no significant difference between the two groups (P> 0.05). The memory retention rate of low-dose lead-Ⅰ group and high-dose lead-Ⅰ group were higher than that of lead model group (P <0.05, P <0.001). The memory retention rate of low-dose lead-Ⅰ group was higher than that of EDTA group (P <0.01). The retention rate of low-dose lead-1 group was higher than that of middle- The apoptosis index of hippocampus in the lead group decreased significantly (P <0.05, P <0.01). Conclusion Jiangzao Ⅰ can reduce the levels of lead and lead in pups exposed to low levels of lead and significantly improve the learning and memory abilities of pups exposed to low levels of lead.