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在紫草色素生产培养基中添加琼脂糖、人参多糖、海藻多糖、黄原胶、卡拉胶和淀粉等6种多糖类物质,接入紫草细胞后置于25℃黑暗条件下振荡培养20d,结果表明:试验中所选用的6种多糖类物质对紫草色素的产生均具有一定的刺激作用,其中以0.05%琼脂糖的作用效果为最佳,紫草色素含量为20.77%,比对照提高46.89%。此外试验亦对琼脂糖对紫草色素生产及分泌特征、培养液中蔗糖浓度变化以及pH值变化情况进行了研究。
Six kinds of polysaccharides, such as agarose, ginseng polysaccharides, seaweed polysaccharides, xanthan gum, carrageenan and starch, were added to the purple pigment production medium, and then the purple polysaccharides were added into the purple color pigment production medium and then cultured in the dark at 25 ° C. for 20 days The results showed that all of the six polysaccharides selected in the experiment had a certain stimulating effect on the production of comfrey pigment. The effect of 0.05% agarose was the best, and the content of comfrey pigment was 20. 77%, 46.89% higher than the control. In addition, the experiment also studied the characteristics of agarose production and secretion of comfrey pigment, sucrose concentration in culture broth and pH value.