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目的:初步建立人鼠嵌合乙肝动物模型。方法:将来源于人并嵌合有乙型肝炎病毒(HBV)全基因序列的肝癌细胞(HepG2.2.15,以下简称“2215细胞”)经孕鼠子宫注射到孕期为15～18天的胎鼠腹中,诱导胎鼠对2215细胞产生免疫耐受,且在胎鼠出生后24h内经脾再次移植2215细胞。ELISA法检测不同时相点大鼠血清中的表面抗原(HBsAg)。结果:对照组与正常组大鼠的各时相点的HBsAg表达均为阴性,模型组大鼠第3、4、5、6、7、8、9、10、11、12、13、14、15、16周龄的HBsAg阳性率分别为60.5%、60.5%、58.1%、58.1%、48.8%、46.5%、47.6%、42.9%、35.7%、31.0%、31.7%、29.3%、19.5%、12.2%。结论:模型组中部分大鼠(48.27%)血清HBsAg的表达随着鼠龄的增加而减退,而另一部分大鼠(12.20%)可以将这种阳性表达持续到16周,表明该模型有长期表达的可能性。
Objective: To establish a human chimeric hepatitis B animal model. Methods: Hepatocarcinoma cells (HepG2.2.15, hereinafter referred to as “2215 cells”) derived from human and chimeric with the complete genome of hepatitis B virus (HBV) were injected into the uterus of pregnant mice for 15 to 18 days Fetal abdomen, inducing fetal mice immune tolerance to 2215 cells, and transplanted 2215 cells in the spleen of the fetus after birth within 24h. ELISA method was used to detect serum antigens (HBsAg) in rat serum at different time points. Results: The expression of HBsAg in the control group and the normal group was negative at each time point, and in the model group, the 3rd, 4th, 5th, 6th, 7th, 8th, 9th, 10th, The positive rates of HBsAg in 15 and 16 weeks old were 60.5%, 60.5%, 58.1%, 58.1%, 48.8%, 46.5%, 47.6%, 42.9%, 35.7%, 31.0%, 31.7%, 29.3%, 19.5% 12.2%. Conclusion: The expression of HBsAg in serum of some rats (48.27%) in the model group decreased with the increase of the age of the mice, while the expression of HBsAg in the other rats (12.20%) lasted for 16 weeks, indicating that the model has long-term The possibility of expression.