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目的:研究巨噬细胞对卵巢癌细胞株SKOV3生物学功能的影响。方法:(1)体外采用IL-4和佛波醇酯(PMA)分别诱导M2和M1型巨噬细胞,流式细胞仪鉴定分型;(2)Tranwell小室建立巨噬细胞与卵巢癌细胞SKOV3体外非接触式共培养模型。比较共培养后,SKOV3的增殖和凋亡、迁移和侵袭的变化。MTT法检测增殖;流式细胞仪Annexin V-FITC/PI双染检测凋亡;Transwell检测侵袭和迁移。结果:(1)IL-4诱导的巨噬细胞高表达CD163,为M2型,PMA诱导组高表达HLA-DR,为M1型。SKOV3和普通巨噬细胞共培养后,巨噬细胞CD163高表达。(2)SKOV3的增殖和凋亡:M2共培养组SK-OV3的增殖活性显著高于M1共培养组和普通共培养组(P<0.05)。M2共培养组SKOV3的凋亡率显著低于M1共培养组和普通共培养组(P<0.05)。(3)SKOV3的迁移和侵袭:M2共培养组SKOV3的侵袭能力显著强于M1共培养组和普通共培养组(P<0.01)。M2共培养组SKOV3的迁移能力显著强于M1共培养组和普通共培养组(P<0.05)。结论:共培养卵巢癌细胞使巨噬细胞M2表型极化。M2型巨噬细胞促进卵巢癌细胞增殖,提高侵袭、迁移能力,减少凋亡,而M1型起相反作用。
Objective: To study the effect of macrophages on the biological function of ovarian cancer cell line SKOV3. Methods: (1) M2 and M1 macrophages were induced by IL-4 and phorbol ester (PMA) in vitro respectively, and then identified by flow cytometry. (2) Tranwell cells were used to establish the macrophages and ovarian cancer cells SKOV3 In vitro non-contact co-culture model. The changes of proliferation and apoptosis, migration and invasion of SKOV3 were compared after co-culture. Proliferation was detected by MTT assay. Apoptosis was detected by flow cytometry Annexin V-FITC / PI double staining. Transwell assay was used to detect the invasion and migration. Results: (1) CD163 was highly expressed in IL-4-induced macrophages, which was type M2. HLA-DR was highly expressed in PMA induction group and was type M1. After co-cultured with SKOV3 and normal macrophages, CD163 macrophages were highly expressed. (2) Proliferation and apoptosis of SKOV3: The proliferation activity of SK-OV3 in M2 co-culture group was significantly higher than that in M1 co-culture group and common co-culture group (P <0.05). The apoptosis rate of SKOV3 in M2 co-culture group was significantly lower than that in M1 co-culture group and common co-culture group (P <0.05). (3) The migration and invasion of SKOV3: The invasive ability of SKOV3 in M2 co-culture group was significantly stronger than that in M1 co-culture group and common co-culture group (P <0.01). The migration ability of SKOV3 in M2 co-culture group was significantly stronger than that in M1 co-culture group and common co-culture group (P <0.05). Conclusions: Co-culturing ovarian cancer cells to polarize the M2 phenotype of macrophages. M2 macrophages promote ovarian cancer cell proliferation, increase invasion, migration and reduce apoptosis, while M1 plays the opposite role.