论文部分内容阅读
目的:探讨非小细胞肺癌(NSCLC)原发灶与相应转移淋巴结中EGFR表达丰度,及其与临床病理因素的相关性。方法:选取20例病理确诊为NSCLC的患者作为研究对象,其中腺癌10例,鳞癌6例,腺鳞癌2例,大细胞癌2例。应用免疫组化二步法检测原发灶和转移淋巴结EGFR表达,并应用SPSS 13.0统计软件分析其与临床病理因素的相关性。结果:原发灶EGFR阳性率为70.0%(14/20),其中弱阳性2例,中度阳性8例,强阳性4例;转移淋巴结阳性率为55.0%(11/20),弱阳性3例,中度阳性4例,强阳性4例。原发灶EGFR表达丰度与病理类型有关(P=0.018),其中腺癌EGFR表达丰度与鳞癌表达丰度差异有统计学意义,P=0.003;而转移淋巴结EGFR表达丰度与性别、年龄、TNM分期和病理类型均无关,P值均>0.05。原发灶和转移淋巴结中EGFR蛋白表达丰度没有相关性,P=0.081。结论:NSCLC原发灶与转移淋巴结的EGFR阳性表达丰度无相关性,原发灶的EGFR表达丰度与病理类型相关。
Objective: To investigate the expression abundance of EGFR in primary tumor and its corresponding metastatic lymph nodes of non-small cell lung cancer (NSCLC) and its correlation with clinicopathological factors. Methods: Twenty patients with pathologically diagnosed as NSCLC were selected as study subjects, including 10 adenocarcinoma, 6 squamous cell carcinoma, 2 adenosquamous carcinoma and 2 large cell carcinoma. The expression of EGFR in primary tumor and metastatic lymph nodes was detected by immunohistochemical two-step method. SPSS 13.0 software was used to analyze the correlation between EGFR and clinical pathological factors. Results: The positive rate of EGFR in primary tumor was 70.0% (14/20), of which 2 were weakly positive, 8 moderately positive and 4 were strongly positive. The positive rate of metastatic lymph node was 55.0% (11/20) and weakly positive For example, 4 were moderately positive and 4 were strongly positive. The abundance of EGFR in primary tumor was related to the pathological type (P = 0.018). There was a significant difference between the expression of EGFR and the expression of squamous cell carcinoma in adenocarcinoma (P = 0.003) Age, TNM stage and pathological type had no correlation, P values were> 0.05. There was no correlation between the abundance of EGFR protein in primary and metastatic lymph nodes, P = 0.081. Conclusion: There is no correlation between the expression of EGFR and the abundance of EGFR in the primary and metastatic lymph nodes of NSCLC. The abundance of EGFR in the primary tumor is correlated with the pathological type.