,Interaction between M-CSF and IL-10 on productions of IL-12 and IL-18 and expressions of CD14, CD23

• 作 者: Xiao-hui JI Ting YAO Jun-chuan QIN Shu-kui WANG Hui-juan WANG Kun YAO
• 机 构: Microbiology and Immunology Institute of Nanjing Medical University,Nanjing,210029“,”National Key La
• 出 处: 中国药理学报（英文版）
• 发表日期: 2004年10期
• 关键词: macrophage colony-stimulating factor interleukin-10 monocytes cytokines membrane

AIM: To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS:Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, the culture supeatants and cells were separated and collected. IL-12P40 and IL-18 levels in the supeatants were determined by ELISA and the percentages of CD14, CD23, and CD64 positive cells were examined by flow cytometry. RESULTS: (1) IL-10 decreased M-CSF-induced IL-18 levels, while M-CSF further reduced IL-12P40 level in the culture supeatants of IL-10-treated monocytes; (2) IL-10 alone had no effect on the percentage of CD14-positive cells, but further increased the percentage of CD14-positive cells induced by M-CSF; M-CSF alone had no effect on the percentage of CD64-positive cells, but further increased the percentage of CD64-positive cells induced by IL-10; (3) IL-10 decreased the percentage of CD23-positive cells induced by M-CSF.CONCLUSION: Between M-CSF and IL-10, there were antagonistic effects on inducing IL-18 and CD23 expressions by monocytes; there were also synergistic effects on inhibiting IL-12P40 production and inducing CD 14 and CD64 expressions by monocytes.