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目的分析miR-886-3p在肾细胞癌与相应癌旁正常组织中的表达情况,为进一步研究miR-886-3p在肾癌发生发展中的作用奠定基础,并初步探讨其临床意义。方法收集48例肾癌手术患者癌组织和癌旁正常组织标本,分别提取组织中的总RNAs,通过逆转录获得cDNA,实时定量PCR法分析miR-886-3p在肾癌组织及癌旁正常组织中的表达情况,并分析其表达量与患者临床病理各指标之间的相关性。结果与相应癌旁正常组织对比,miR-886-3p在肾癌组织中存在显著高表达,48例标本中,有35例(72.92%)miR-886-3p表达上调,13例(27.08%)表达下调,差异有统计学意义(P=0.000),平均升高5.125倍。但miR-886-3p的表达水平与肾癌患者年龄、性别、病理类型、临床TNM分期(主要为T1N0M0和T2N0M0期)没有关系(P>0.05)。结论 miR-886-3p在肾癌组织中存在显著高表达,提示它可能与肾癌的发生与进展有关,可能成为指导肾癌诊断、治疗乃至预后判断的新的生物标志物。
Objective To analyze the expression of miR-886-3p in renal cell carcinoma and adjacent normal tissues, and to lay a foundation for further study on the role of miR-886-3p in the occurrence and development of renal cell carcinoma and to investigate its clinical significance. Methods 48 cases of renal cell carcinoma and adjacent normal tissue samples were collected from the tissues of total RNAs were extracted by reverse transcription of cDNA, real-time quantitative PCR analysis of miR-886-3p in renal cell carcinoma and adjacent normal tissue In the expression of the situation, and analyze the relationship between the expression and clinical pathology of the various indicators. Results The miR-886-3p expression was significantly higher in renal cell carcinoma than in corresponding adjacent normal tissues. MiR-886-3p expression was up-regulated in 35 (72.92%) and 13 (27.08%) of the 48 specimens, The expression was down, the difference was statistically significant (P = 0.000), an average of 5.125 times higher. However, the expression level of miR-886-3p was not associated with age, sex, pathological type, clinical stage of TNM (mainly T1N0M0 and T2N0M0) in patients with renal cell carcinoma (P> 0.05). Conclusions miR-886-3p is highly expressed in human renal cell carcinoma, suggesting that miR-886-3p may be associated with the development of renal cell carcinoma and may be a new biomarker to guide the diagnosis, treatment and prognosis of renal cell carcinoma.