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本文报道茶碱与苯巴比妥（ＰＢ）、苯妥英（ＤＰＨ）、卡马西平（ＣＢＺ）两类（４种）不同药物血浓度的ＨＰＬＣ同时测定法．采用国产色谱柱ＹＷＧＣ１８（４．６×２５０ｍｍ），检测波长为２５４ｎｍ，流动相为甲醇－水（５０：５０，ｖ／ｖ），流速：１ｍｌ／ｍｉｎ，以４－氨基安替匹林作内标，各药物的平均回收率分别为茶碱９９．０２％，ＰＢ１００．７３％，ＤＰＨ１０１．０％，ＣＢＺ１０１．７７％。对各药血清标准液的峰高比测量的变异系数（ｎ＝１０），分别为茶碱（１０μｇ／ｍｌ）６．３％，ＰＢ（２０μｇ／ｍｌ）６．１％，ＤＰＨ（２０μｇ／ｍｌ）４．０％，ＣＢＺ（１０μｇ／ｍｌ）５．２％。本法具有快速、准确、适用性广的特点，用于治疗药物的监测，效果满意，大大提高了实验室的工作效率。
This paper reports the Simultaneous Determination of Serum Concentrations of theophylline with two different drugs (PB, phenytoin (DPH) and carbamazepine (CBZ)). Using domestic column YWGC18 (4.6 × 250mm), the detection wavelength was 254nm, the mobile phase was methanol-water (50:50, v / v), the flow rate was 1ml / min, The average recoveries of theophylline were 99.02%, PB100.73%, DPH101.0% and CBZ101.77% respectively. The coefficient of variation (n = 10) for the peak-to-height ratio of each drug serum standard was 6.3% for theophylline (10μg / ml), 6.1% for the PB (20μg / ml), DPH ) 4.0%, CBZ (10 μg / ml) 5.2%. This method has the characteristics of fast, accurate and wide applicability for the monitoring of therapeutic drugs with satisfactory results and greatly improves the work efficiency of the laboratory.