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目的通过对新型甲型H1N1流感毒株神经氨酸酶(NA)基因序列的变异分析,揭示毒株NA基因变异与进化。方法检测广东地区分离毒株NA基因核苷酸序列,同时对全球新型甲型H1N1流感毒株NA基因进行检索下载,采用Lasergene 7.1软件比对和分析NA基因核苷酸和氨基酸序列,结合临床资料分析变异基因进化,同时分析糖基化位点。结果 2009年4-12月69株毒株NA基因16个氨基酸位点置换,占3.41%(16/469);NA蛋白主要置换位点在V106I/H和N248D。毒株GD-801-2009(H1N1)和GD-1202-2009的NA基因明显受到的感染宿主选择性作用,而毒株GD-1-2009的NA基因明显受到的感染宿主负选择性作用。与毒株GD-1-2006(H5N1)NA基因比较,毒株GD-801-2009的NA基因插入Nt145-204,导致H1N1NA蛋白增加第49～68位氨基酸(CNQSVITYENNTWVNQTYVN),其中包括4个糖基化位点。结论广东新型甲型H1N1流感毒株NA基因正向不同方向进化;新型甲型H1N1流感NA蛋白新增4个糖基化位点。
Objective To reveal the variation and evolution of NA gene by analyzing the variation of neuraminidase (NA) gene sequence of new type A (H1N1) influenza virus. Methods The nucleotide sequence of NA gene in Guangdong isolates was detected. At the same time, the NA gene of new type A (H1N1) strains was searched and downloaded. The nucleotide and amino acid sequences of NA gene were compared and analyzed by Lasergene 7.1 software. Analysis of variant gene evolution, simultaneous analysis of glycosylation sites. Results The 16 amino acid substitutions of NA genes in 69 isolates from April to December in 2009 accounted for 3.41% (16/469). The main substitution sites for NA protein were V106I / H and N248D. The NA genes of strains GD-801-2009 (H1N1) and GD-1202-2009 were significantly affected by the selective host infection, while the NA gene of strain GD-1-2009 was significantly negatively affected by the host infection. Compared with the GD-1-2006 (H5N1) NA gene of the strain, the NA gene of strain GD-801-2009 was inserted into Nt145-204, resulting in the addition of amino acids 49-68 (CNQSVITYENNTWVNQTYVN) to the H1N1NA protein, including four glycosyl The site. Conclusion The NA gene of new type A (H1N1) strains of Guangdong has been evolving in different directions. There are four additional glycosylation sites in the novel NA protein of influenza A (H1N1).