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本文报道一种简便、快速、准确和广泛用于测定抗坏血酸的分光光度法.其原理是抗坏血酸与1,10-菲咯啉和铁(Ⅲ)离子络合生成三-(1,10-菲咯啉)-铁(Ⅱ)络合物(试亚铁灵),其最大吸收波长为510nm,一般赋形剂和其它化合物对测定均无干扰,最佳pH值为1.5～6.5.试剂:(1)20mg/L抗坏血酸标准液;(2)1,10-菲咯啉-铁(Ⅲ)显色剂:取0.198g 1,10-菲咯啉单水合物、1mol/L盐酸2ml和0.16g硫酸铁铵混合,用水稀释至100ml.纯抗坏血酸的测定;精密称取样品水溶液(含抗
A simple, rapid, accurate and widely used spectrophotometric method for the determination of ascorbic acid is reported in this paper. The principle is that ascorbic acid is complexed with 1,10-phenanthroline and iron (III) ions to form tris- (1,10-phenanthroline The maximum absorption wavelength is 510nm, the general excipients and other compounds have no interference with the determination, the best pH value is 1.5 ~ 6.5. Reagents: (1 ) 20mg / L ascorbic acid standard solution; (2) 1,10-phenanthroline-iron (III) developer: 0.198g of 1,10-phenanthroline monohydrate, 2ml of 1mol / L hydrochloric acid and 0.16g of sulfuric acid Ferric ammonium mixed, diluted with water to 100ml Determination of pure ascorbic acid; accurately weighed sample aqueous solution (containing anti