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采用根袋法盆栽试验,研究分别接种活菌量浓度为1×1010CFU.kg-1(处理A)和2×1010CFU.kg-1(处理C)的胶质芽胞杆菌,对根际土壤镉含量和土壤酶活性的影响.结果表明,处理A、C对根际土壤镉的去除率分别达到37.62%、38.27%,是对照(24.47%)的1.54、1.56倍.胶质芽胞杆菌处理后土壤酶活性均有所增强,土壤脲酶、磷酸酶、过氧化氢酶活性在根际土壤大于非根际,随着时间延长土壤磷酸酶活性逐渐升高,脲酶和过氧化氢酶呈现先增高后降低的变化趋势,而脱氢酶活性在非根际土壤大于根际土壤,也呈现先增高后降低的变化趋势.对照组及处理A、C组中根际Cd含量与土壤磷酸酶、脲酶呈现显著或极显著负相关.研究表明,施入胶质芽胞杆菌不仅可以提高超富集植物对土壤Cd的净化效果而且对土壤脲酶、磷酸酶、过氧化氢酶及脱氢酶均有一定的积极作用,其结果可为该菌辅助植物修复镉污染土壤的机制性研究提供理论指导.
The pot experiment with root bag method was used to study the effects of inoculating Bacillus subtilis on the soil with 1 × 1010CFU.kg-1 (treatment A) and 2 × 1010CFU.kg-1 (treatment C) And soil enzyme activities.The results showed that the removal rates of cadmium in rhizosphere soil were 37.62% and 38.27%, respectively, which were 1.54 and 1.56 times that of the control (24.47% The activities of soil urease, phosphatase and catalase were higher in the rhizosphere soil than in the non-rhizosphere soil. The activities of soil phosphatase increased with time, and the urease and catalase increased first and then decreased While the activity of dehydrogenase in the rhizosphere soil was higher than that in the rhizosphere soil at the beginning and then decreased.The content of Cd in the rhizosphere and the content of phosphatase and urease in the control and treatment groups A and C were significantly Significant negative correlation.Studies showed that the application of Bacillus gliadin not only can improve the purification effect of hyperaccumulator on soil Cd, but also have some positive effects on soil urease, phosphatase, catalase and dehydrogenase, The result may be that the bacteria assist the plant in repairing cadmium contaminated soil Provide theoretical guidance mechanistic studies.