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目的:探讨超排卵周期中获得的未成熟卵母细胞的利用价值及人工辅助激活能否改善源于此类卵母细胞的胚胎的发育结局。方法:对226枚超排卵周期中获得的未成熟卵母细胞行体外成熟(IVM)培养,发育至MⅡ期卵母细胞行卵胞浆内单精子显微注射(ICSI)授精,受精后卵母细胞随机分成非激活组和激活组,非激活组卵母细胞ICSI后不作任何处理直接移入G-1中培养,激活组卵母细胞ICSI后入7%无水乙醇作用6 min(即人工辅助激活),比较两组受精、卵裂、优质胚胎、囊胚及优质囊胚形成情况。结果:未激活组和激活组体外成熟率分别为83.9%(94/112)和82.5%(94/114)、受精率分别为80.9%(76/94)和76.6%(72/94)、优质胚胎率分别为14.3%(10/70)和25.0%(18/72),囊胚形成率分别为8.6%(6/70)和16.7%(12/72),两组间差异无统计学意义(P>0.05),但卵裂率激活组(100%,72/72)高于未激活组(92.1%,70/76),差异有统计学意义(P<0.05),优质囊胚率激活组(83.3%,10/12)明显高于未激活组(0%,0/6),差异有统计学意义(P<0.01)。结论:超排卵周期中的未成熟卵母细胞经体外培养成熟可获得比较满意的受精率及早期胚胎发育潜能,人工辅助激活技术为超排卵周期中获取的未成熟卵母细胞的充分利用提供一种新的思路
OBJECTIVE: To explore whether the utilization value of immature oocytes obtained during superovulation cycle and artificial-assisted activation can improve the developmental outcome of embryos derived from these oocytes. Methods: The immature oocytes obtained during the 226 superovulation cycles were cultured in vitro (IVM), and were fertilized by intracytoplasmic sperm injection (ICSI) with M Ⅱ oocytes. After fertilization, The cells were randomly divided into inactive group and active group. ICSI of non-activated group was transplanted into G-1 without ICSI treatment, ICOS of activated group was injected with 7% ethanol for 6 min (artificial stimulation ). The fertilization, cleavage, high quality embryo, blastocyst and high quality blastocyst formation were compared between the two groups. RESULTS: In vitro maturation rates were 83.9% (94/112) and 82.5% (94/114), respectively. The fertilization rates were 80.9% (76/94) and 76.6% (72/94) respectively, The embryo rates were 14.3% (10/70) and 25.0% (18/72), respectively. The blastocyst formation rates were 8.6% (6/70) and 16.7% (12/72), respectively. There was no significant difference between the two groups (P> 0.05). However, the rate of cleavage rate activation group (100%, 72/72) was higher than that of inactive group (92.1%, 70/76), the difference was statistically significant (P <0.05) Group (83.3%, 10/12) was significantly higher than the inactive group (0%, 0/6), the difference was statistically significant (P <0.01). CONCLUSIONS: Immature oocytes in the superovulation cycle can be matured in vitro to obtain satisfactory fertilization rate and early embryonic development potential. The artificial assisted activation technique provides a full utilization of the immature oocytes obtained in the superovulation cycle Kind of new ideas