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目的:研究Apogossypolone(ApoG2)对人乳腺癌细胞系MCF-7体外的增殖抑制和诱导凋亡作用。方法:采用MTT法和平板克隆形成实验检测ApoG2作用于MCF-7细胞后,对其体外增殖和细胞克隆形成能力的影响;Hoechst33258荧光染色法观察ApoG2(20μmol/L)作用48h后,细胞凋亡的形态学变化;FCM检测ApoG2作用于MCF-7细胞后的细胞凋亡率以及细胞周期的变化。结果:ApoG2药物在浓度为5μmol/L、作用14d后就能对MCF-7细胞增殖起到明显的抑制作用,且呈剂量和时间依赖性;平板克隆实验表明,经不同浓度(5、10和20μmol/L)ApoG2作用14d后,对MCF-7细胞克隆形成能力呈现明显的抑制作用,且同样呈剂量和时间依赖性。Hoechst33258荧光染色结果显示,ApoG2与MCF-7细胞共培养72h后,细胞呈现明显的核固缩和碎裂、染色质凝集和凋亡小体形成等细胞凋亡现象。FCM检测结果显示,20μmol/L ApoG2作用72h后,细胞凋亡率较对照组升高,而且S期和G2/M期细胞比例较对照组升高(P<0.05)。结论:ApoG2能够抑制人乳腺癌细胞系MCF-7的增殖和克隆形成,阻滞细胞周期,诱导细胞凋亡。
AIM: To investigate the proliferation and apoptosis-inducing effects of Apogossypolone (ApoG2) on human breast cancer cell line MCF-7 in vitro. Methods: The effect of ApoG2 on proliferation and cell clone formation ability was detected by MTT assay and plate clone formation assay. The apoptosis of MCF-7 cells was detected by Hoechst33258 fluorescence staining after 48h treatment with ApoG2 (20μmol / L) Morphological changes were observed by flow cytometry. Apoptosis rate and cell cycle of ApoG2 cells treated with ApoG2 were detected by FCM. Results: ApoG2 could significantly inhibit the proliferation of MCF-7 cells at a concentration of 5μmol / L for 14 days, and dose-dependent and time-dependent manner. The results of plate cloning experiments showed that after treated with different concentrations (5, 10 and 20μmol / L) ApoG2 for 14d showed a significant inhibitory effect on the colony formation ability of MCF-7 cells, and also in a dose-and time-dependent manner. Hoechst33258 staining results showed that after co-cultured with ApoG2 and MCF-7 cells for 72h, the cells showed obvious phenomena of nuclear condensation and fragmentation, chromatin condensation and apoptotic body formation. The result of FCM showed that the apoptotic rate of ApoG2 cells treated with 20μmol / L ApoG2 for 72h was higher than that of control group, and the percentage of cells in S phase and G2 / M phase was higher than that of control group (P <0.05). Conclusion: ApoG2 can inhibit the proliferation and colony formation of human breast cancer cell line MCF-7, arrest cell cycle and induce apoptosis.