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目的:观察钙调磷酸酶B同源蛋白2（CHP2）在临床胃癌组织中的表达和恶性表型作用并分析其临床意义。方法:应用临床胃癌组织芯片（297例胃癌和198例对照良性胃黏膜组织），均来自南通大学附属医院生物样本库。经免疫组织化学法检测胃癌组织中CHP2蛋白表达水平，统计分析CHP2蛋白表达水平与胃癌患者临床特征及预后的关系。在胃癌细胞系中分别进行CHP2抑制和过表达细胞学实验，用细胞划痕实验、Transwell实验和CCK-8法检测胃癌细胞恶性表型变化。结果:CHP2蛋白在胃癌组织中高表达（204/297，68.7%），高于非癌的手术切缘组织（31/91，34.1%）、慢性胃炎组织（13/22，59.1%）、肠化胃黏膜组织（13/38，34.2%)、低级别上皮内瘤变胃黏膜组织（12/30，40.0%）以及高级别上皮内瘤变胃黏膜组织（7/17，41.2%）。胃癌组织中CHP2蛋白高表达与胃癌有转移、TNM分期晚有关（n P<0.05），多因素分析显示，CHP2蛋白高表达，TNM分期是胃癌患者预后的独立指标（n P<0.05）。胃癌细胞HGC-27在下调CHP2表达后，增殖、迁移能力下降（n P<0.05）；胃癌细胞AGS在上调CHP2表达后，增殖、迁移能力增强（n P<0.05）。n 结论:在胃癌发展中，CHP2起到促进增殖和转移作用，可以作为胃癌患者预后的分子标志物以及靶向治疗的潜在靶点。“,”Objective:To study the expression and clinical significance of calcineurin B homologous protein 2 (CHP2) in gastric cancer (GC) and its effect on malignant phenotype of GC cells.Methods:The protein expression of CHP2 in 297 GC tissue and 198 normal gastric tissue samples were detected by immunohistochemistry. The relationship between the expression of CHP2 and clinicopathologic parameters of GC were analyzed. CHP2-overexpression plasmids and CHP2-interference plasmids were transfected into GC cell lines respectively. Wound healing assay and Transwell experiment was used to detect the invasion and migration ability of GC cells, and cell counting kit-8 (CCK-8) method was used to detect the proliferation ability of GC cells.Results:The positive expression rate of CHP2 in GC was 68.7% (204/297), which was higher in benign margin (34.1%) (31/91), chronic gastritis (59.1%) (13/22), intestinal metaplasia (34.2%) (13/38), low-grade intraepithelial neoplasia (40.0%) (12/30) and high-grade intraepithelial neoplasia (41.2%)(7/17). The positive expression of CHP2 was correlated with tumor, node and metastasis (TNM) stage, lymph node metastasis and distant metastasis (all n P0.05). The results of multivariate analysis showed that high expression of CHP2 and TNM stage were both independent parameters for predicting GC patient prognosis (bothn P<0.05). Interference of CHP2 expression in HGC-27 cells suppressed proliferation and migration significantly (n P<0.05). However, over-expression CHP2 in AGS cells promoted proliferation, and migration significantly (n P<0.05).n Conclusion:CHP2 plays an important role in the development of GC, which is expected to be a molecular marker for patient prognosis and a potential target of targeted therapy for GC patients.