论文部分内容阅读
目的:探讨醒脑静合生脉注射液对大鼠脑出血后脑组织内水通道蛋白-4(AQP4)表达的影响。方法:将SD大鼠随机分为脑出血组(ICH)、生理盐水组(NS)、醒脑静合生脉注射液治疗组(XNJSM)、水蛭素治疗组(HIR),每组10只大鼠。采用自体不凝血注入法复制脑出血模型,造模后6 h,XNJSM组ip醒脑静合生脉注射液2 mL.kg-1+10 mL.kg-1(按1∶5比例混合),1次/d,连续3 d;HIR组给予脑内注入水蛭素10 U(5μL)1次。术后72 h取脑组织,HE染色观察各组血肿周围神经细胞形态学改变,免疫组化法及免疫印迹(Western blot)法检测各组血肿周围脑组织AQP4的表达。结果:脑出血后72 h脑组织内AQP4阳性细胞及蛋白表达与NS组比较明显增加(P<0.05),XNJSM组、HIR组脑组织病理形态明显改善,脑组织AQP4表达减少,与ICH组比较差异均有显著性(P<0.05)。结论:醒脑静合生脉注射液能够有效抑制大鼠脑出血后AQP4蛋白的表达,减轻脑水肿,对脑出血后脑组织发挥保护作用。
Objective: To investigate the effect of Xingnaojing Jinghe Shengmai injection on aquaporin-4 (AQP4) expression in brain tissue after intracerebral hemorrhage in rats. Methods: SD rats were randomly divided into intracerebral hemorrhage (ICH) group, NS group, XNJSM group, HIR group, 10 rats in each group . The model of intracerebral hemorrhage was established by autologous non-coagulation infusion injection. After 6 h of modeling, the rats in XNJSM group were treated with 2 mL.kg-1 + 10 mL.kg-1 ip (1: 5) Times / d for 3 consecutive days. HIR group was injected with 10 U (5 μL) hirudin once a day. The brain tissue was harvested at 72 h after operation. The morphological changes of the peripheral nerve cells in the hematoma were observed by HE staining. The expression of AQP4 in the perihematomal brain tissue was detected by immunohistochemistry and Western blot. Results: Compared with NS group, the expression of AQP4 positive cells and protein in brain tissue increased significantly at 72 h after intracerebral hemorrhage (P <0.05). The pathological changes of brain tissue in XNJSM group and HIR group were significantly reduced and AQP4 expression decreased The difference was significant (P <0.05). Conclusion: Xingnaojing Jinghe Shengmai injection can effectively inhibit the expression of AQP4 protein after cerebral hemorrhage in rats, reduce cerebral edema, and play a protective role in cerebral tissue after intracerebral hemorrhage.