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目的:评估半边旗提取物5F-Na盐溶液对肝细胞癌细胞株的增殖抑制作用。方法:采用MTT法,观察不同浓度的半边旗提取物物5F-Na盐溶液分别作24、48、72h对三种不同类型肝细胞癌细胞株(HepG2,Hep3B,BEL-7402)生长增殖的影响。结果:不同浓度的半边旗提取物5F-Na盐溶液分别对3种肝癌细胞作用24、48、72h后,所有的肝癌细胞株生长增殖均受到不同程度的抑制(P<0.05),5F-Na盐溶液对HepG2,Hep3B,BEL-7402作用72h后的IC50分别是20.67,16.74,16.09mg/L。结论:半边旗提取物5F-Na盐溶液能有效抑制肝细胞癌细胞株的体外增殖。
Objective: To evaluate the inhibitory effect of 5F-Na salt extract of Pteris semipinnata on the proliferation of hepatocellular carcinoma cell lines. Methods: MTT assay was used to observe the effect of different concentrations of 5F-Na salt extract of Pteris semipinnata on the growth and proliferation of three different types of hepatocellular carcinoma cell lines (HepG2, Hep3B and BEL-7402) for 24, 48 and 72 hours respectively . Results: The proliferation and proliferation of all hepatocellular carcinoma cell lines were inhibited to varying degrees (P <0.05) after treated with different concentrations of 5F-Na salt solution of Pteris semipinnata for 24 hours, 48 hours and 72 hours, respectively. The levels of 5F-Na The IC50 of salt solution on HepG2, Hep3B and BEL-7402 after 72h were 20.67, 16.74 and 16.09 mg / L, respectively. Conclusion: The extract of Pteris semipinnata 5F-Na can effectively inhibit the proliferation of hepatocellular carcinoma cell lines in vitro.