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目的:观察大黄虫丸含药血清对人肺腺癌细胞A549增殖的影响。方法:12只SD大鼠随机分为大黄虫丸组(DHZCW组)、鸦胆子组、生理盐水组,按常规方法制备含药血清。然后以细胞培养液将含药血清稀释成不同的浓度(40%、20%、10%、5%),采用MTT法检测不同浓度含药血清对A549细胞增殖的影响。结果:与生理盐水组比较,20%、40%DHZCW组和20%、40%鸦胆子组OD值降低,差异有统计学意义(P<0.05);与鸦胆子组比较,40%DHZCW组OD值降低,差异有统计学意义(P<0.05)。结论:大黄虫丸含药血清与鸦胆子油口服乳液含药血清均具有体外抑制人肺腺癌A549细胞增殖的作用。
Objective: To observe the effect of rhubarb Zhechong pill containing serum on the proliferation of human lung adenocarcinoma A549 cells. Methods: Twelve SD rats were randomly divided into DHZCW group, Brucea javanicae group and normal saline group. The drug-containing serum was prepared by routine method. Then the drug-containing serum was diluted to different concentrations (40%, 20%, 10%, 5%) with cell culture medium. The effects of different concentrations of serum on the proliferation of A549 cells were detected by MTT assay. Results: Compared with saline group, the OD values of 20%, 40% DHZCW group and 20%, 40% Brucea Javanica group were significantly lower than those of normal saline group (P <0.05) Value decreased, the difference was statistically significant (P <0.05). Conclusion: The drug serum containing Dahuang Zhechong pill and the oral emulsion of Brucea javanica oil all have the effect of inhibiting the proliferation of human lung adenocarcinoma A549 cells in vitro.