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采用离体平皿测定法,分别对黄河流域8个不同抗病性的常规棉花品种,进行种子内部和外部带菌检测,根据分离纯化的真菌核糖体ITS(Internal transcribed spacer)序列进行分类鉴定。结果表明,棉花种子外部携带了10种真菌,内部寄藏了7种,优势菌群是木贼镰刀菌(Fusarium equiseti)和滕仓赤霉菌(Gibberella intermedia)。部分品种的种子外部检测到了黄萎病菌(Verticillium dahliae),但并未发现枯萎病菌(Fusarium oxysporum)。另外在种子内部均没有检测到棉花枯黄萎病菌。
Eight inbred cotton cultivars with different disease resistances in the Yellow River Basin were tested for their internal and external fungistasis using in vitro plate assay. The isolates were identified according to the sequence of ITS (Internal transcribed spacer) isolated and purified. The results showed that 10 kinds of fungi were carried on the outside of cotton seeds and 7 were stored on the inside of the cotton seeds. The dominant bacteria were Fusarium equiseti and Gibberella intermedia. Some varieties of Verticillium dahliae were detected outside the seeds, but Fusarium oxysporum was not found. In addition, no cotton wilt was detected in the seeds.