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以２０条／只和１００条／只剂量的尾蚴感染家兔，并于第９ｗｋ用吡喹酮对约半数家兔进行一次性灌胃治疗，依此建立日本血吸虫感染模型。用分别定位于日本血吸虫表皮膜、肠道上皮和虫卵的单克隆抗体建立直接斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）并应用该法分别检测感染家兔血中３类循环抗原、膜相关抗原（ＭＡＡ），肠相关抗原（ＧＡＡ）和可溶性虫卵抗原（ＳＥＡ），观察循环抗原滴度水平和日本血吸虫感染度之间的关系。结果发现，当用Ｄｏｔ－ＥＬＩＳＡ法检测３类循环抗原时，２０条尾蚴感染组与１００条尾蚴感染组家兔不论治疗与否，血中３类循环坑原滴度水平无显著性差异。因此Ｄｏｔ－ＥＬＩＳＡ法检测循环抗原不适宜于作为日本血吸虫感染度的判断方法
Rabbits were infected with 20 cercariae per 100 rabbits and only one half dose of praziquantel was administrated on the 9th week to establish a model of Schistosoma japonicum infection. Dot-ELISA (Dot-ELISA) was established with monoclonal antibodies targeting Schistosoma japonicum epidermal membrane, intestinal epithelium and eggs respectively, and the method was applied to detect the circulating circulating antigens (MAA), gut-associated antigen (GAA) and soluble egg antigen (SEA), and to observe the relationship between circulating antigen titer and infection rate of Schistosoma japonicum. The results showed that there was no significant difference in the titers of all the three kinds of circulating kits between 20 cercariae infected groups and 100 cercariae infected rabbits, regardless of whether they were treated or not, using Dot-ELISA to detect three kinds of circulating antigens. Therefore, Dot-ELISA detection of circulating antigen is not suitable as a method of judging the degree of infection of Schistosoma japonicum