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将邻联茴香胺(ODA)_H2O2_辣根过氧化物酶(HRP)偶合反应伏安酶联免疫分析新体系,应用于植物病毒葡萄扇叶病毒(grapevinefanleafvirus,GFV)、番茄不孕病毒(tomatoaspermyvirus,TAV)和马铃薯Y病毒(potatovirusY,PVY)的测定。该法对以上各种植物病毒叶澄清液的测定的稀释比均高于酶联免疫吸附分析(ELISA)法,检测范围均宽于ELISA法。
The new system of voltammetric enzyme-linked immunosorbent assay (MIA) coupled with o-dianisidine (ODA) _H2O2_ horseradish peroxidase (HRP) was applied to the phytoestroies of grapevinefanleafvirus (GFV), tomatoaspermyvirus TAV) and potato virus Y (PVY). The method of dilution of the above-mentioned various plant virus leaf serum was higher than the enzyme-linked immunosorbent assay (ELISA) method, the detection range was wider than the ELISA method.