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目的:探讨益母草总碱部位和益母草注射液总碱部位对大鼠体外凝血功能和血小板聚集的影响。方法:首先以酸提取大孔树脂纯化制备益母草总碱(YMC-TA)和益母草注射液总碱(YMCJ-AT),采用HPLC法测定其所含水苏碱、葫芦巴碱含量;然后采用全自动血凝仪测定5.419、2.709、1.355 mg/m L的YMC-TA和10.836、5.419、2.709 mg/m L的YMCJ-AT对体外凝血功能(PT、APTT、TT、FBG)影响,25、18.75、14.06 mg/m L的YMC-TA、YMCJ-AT对二磷酸腺苷(ADP)诱导的体外血小板聚集率的影响。结果:YMC-TA和YMCJ-TA中均含有水苏碱和葫芦巴碱,含量分别是23.77%、34.49%;0.13%、0.14%。二者在试验剂量均能明显缩短体外大鼠血浆PT和APTT时间和降低FBG含量,同时延长TT时间;并且都明显降低ADP诱导的体外大鼠血小板最大聚集率,作用强于阿斯匹林。结论:YMC-TA和YMCJ-TA具有抗凝血和抗血小板聚集作用,但对TT呈促进作用,可能是益母草活血调经、益母草注射液缩宫止血作用的机制之一。
Objective: To investigate the effects of total alkaloids from Motherwort and the total alkaloids of Motherwort injection on the coagulation function and platelet aggregation in rats. Methods: YMC-TA and YMCJ-AT were purified by acid extraction of macroporous resin. The contents of stachydrine and trigonelline were determined by HPLC. The effects of YMC-TA at 5.419, 2.709 and 1.355 mg / mL and YMCJ-AT at 10.836, 5.419 and 2.709 mg / mL on PT, APTT, TT and FBG in vitro were measured by automatic coagulation analyzer. , 14.06 mg / m L YMC-TA, YMCJ-AT on ADP-induced platelet aggregation rate in vitro. Results: Stachydrine and trigonene were all contained in YMC-TA and YMCJ-TA, with contents of 23.77%, 34.49%, 0.13% and 0.14%, respectively. Both of them can obviously shorten the time of PT and APTT, decrease the content of FBG and prolong the time of TT in vitro. Both of them can significantly reduce the maximum platelet aggregation rate induced by ADP in rats, which is stronger than that of aspirin. CONCLUSION: YMC-TA and YMCJ-TA have anticoagulant and anti-platelet aggregation effects, but they have a promoting effect on TT, which may be one of the mechanisms of Leonurice’s activating blood circulation and regulating bleeding and stopping bleeding of Leonurus injection.