动脉内膜损伤模型中合成型平滑肌细胞的培养

• 作 者: 姚玉宇 冷静 尹航 黄峻
• 机 构: 南京医科大学病理学教研室,南京医科大学病理学教研室,南京医科大学第一附属医院心内科!南京210029,南京医科大学第一附属医院心内科!南京210029
• 出 处: 南京医科大学学报
• 发表日期: 2000年01期
• 关键词: 血管平滑肌细胞 细胞培养 鉴定

目的　建立更接近在体情况的合成型血管平滑肌细胞 (VSMC)的培养体系。方法　培养细胞取材于兔动脉内膜损伤后的增生内膜。结果　培养细胞 10天后光镜下表现典型的“峰”、“谷”样排列 ,免疫组化证实是 VSMC,电镜下细胞表现有合成型细胞的特点。结论　直接对合成型 VSMC的培养优于单纯中膜 VSMC培养。

Objective To establish a culture system that is closer to the in vivo situation of synthetic vascular smooth muscle cells (VSMCs). Methods The cultured cells were drawn from the hyperplastic intima after the intimal injury in rabbits. Results After cultured for 10 days, the cells were characterized by “peak” and “valley” arrangement under light microscope. The results of immunohistochemistry showed that the VSMCs were characterized by synthetic cells under electron microscope. Conclusion The cultured VSMC directly outperforms the cultured VSMC.