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为获得冬虫夏草菌固体发酵产分生孢子的最优工艺,以野生分离的冬虫夏草菌为材料,对其固体发酵产分生孢子的培养基及培养条件进行了研究。试验结果表明:泥炭土为最佳基础培养基,该培养基中冬虫夏草菌气生菌丝生长一般,但产分生孢子最多,可达4.2×103个/g;泥炭土培养基中添加0.1‰IAA(吲哚乙酸)、0.1‰IBA(吲哚丁酸)和0.1‰NAA(萘乙酸)能促进冬虫夏草菌气生菌丝的生长和分生孢子的产生,其分生孢子达8.1×103个/g;该基础培养基中,冬虫夏草菌于18℃培养30d后,在10℃、相对湿度45%、蓝光照射进行诱导,分生孢子可达1.0×104个/g。本研究建立了一种大量获取冬虫夏草菌分生孢子的方法,为冬虫夏草繁育奠定了基础。
In order to obtain the best technics of solid fermentation production of conidia of Cordyceps sinensis, the culture medium and culture conditions of conidia produced by solid fermentation were studied using wild Cordyceps sinensis isolated from the wild as raw material. The results showed that peat soil was the best basic medium, the mycelial growth of Cordyceps sinensis was normal, but the number of conidiospores was up to 4.2 × 103 / g. The peat soil added 0.1 ‰ IAA, 0.1 ‰ IBA and 0.1 ‰ NAA could promote the growth of C. aeruginosa mycelia and the production of conidia. The number of conidia reached 8.1 × 103 / g; In the basal medium, Cordyceps sinensis was cultured at 18 ℃ for 30 days and then induced by blue light at 10 ℃ and relative humidity of 45%. The conidia reached 1.0 × 104 / g. This study established a large number of access to Cordyceps sinensis conidia method for the Cordyceps sinensis breeding laid the foundation.