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研究嗜盐杆菌菌株(Halobacillus sp.HSQAY1)发酵液在不同p H、温度和反复冻融条件下对中肋骨条藻(Skeletonema costatum)溶藻活性的环境稳定性;并采用乙醇沉淀、硫酸铵沉淀、超滤和聚丙烯酰胺凝胶电泳(SDS-PAGE)等方法对溶藻物质的理化特性进行了初步探讨和分析。结果表明,该菌株产生的溶藻物质在强酸性条件下(p H=3)丧失活性,p H5~11时的溶藻率在83.4%~98.4%之间;30~110℃时溶藻率在71.7%~94.5%之间,表现出一定的酸碱稳定性和热稳定性,且不受反复冻融(-20℃)影响。溶藻活性物质具有被乙醇和硫酸铵沉淀的特性,其活性组分是多种分子质量>10k Da的蛋白类物质,溶藻活性蛋白粗提物的SDS-PAGE电泳显示,在15~50 k Da间有3条明显的特异蛋白条带。
To study the environmental stability of algae-killing activity of Halobacillus sp. HSQAY1 fermentation broth at different pH, temperature and repeated freeze-thaw cycles, ethanol precipitation, ammonium sulfate precipitation , Ultrafiltration and polyacrylamide gel electrophoresis (SDS-PAGE) and other methods of physical and chemical properties of algae were discussed and analyzed. The results showed that the algicidal substance produced by this strain lost its activity under strong acidic conditions (p H = 3), the rate of algae-lysing was between 83.4% and 98.4% at p H5 ~ 11; Between 71.7% and 94.5%, it shows some pH stability and thermal stability, and is not affected by repeated freezing and thawing (-20 ℃). The algicidal active substance has the characteristics of being precipitated by ethanol and ammonium sulfate, and the active component is a plurality of protein substances with a molecular mass of more than 10 kDa. The SDS-PAGE electrophoresis of the crude algae active protein extract shows that at 15-50 k Da between the three obvious specific protein bands.