论文部分内容阅读
建立了一种简单快速制备交联键合聚丙烯酰胺涂层毛细管电泳柱的方法。在这一方法中，未经处理的毛细管柱直接用含有一定比例的丙烯酰胺、交联剂甲叉基双丙烯酰胺、硅烷化试剂乙烯基三乙氧基硅氧烷以及引发剂偶氮二异丁氰的丙酮溶液进行动态涂渍，再经热处理使单体的交联和聚合物在毛细管壁上的键合反应同时发生。所制柱能够有效地抑制电渗流和蛋白质在毛细管壁上的吸附。碱性蛋白在 ｐＨ ４．０的缓冲液中分离时，获得的平均分离柱效为 ９．１×１０５理论塔板数／米。酸性蛋白在 ｐＨ ８．５的缓冲液中获得的分离柱效为 ２．０×１０４理论塔板数／米。酸碱性蛋白质的迁移时间重现性较好，并且所制柱在 ｐＨ ２．５～ ８．５的范围内表现了较好的稳定性。该方法还具有较高的制柱成功率
A simple and rapid method for preparing crosslinked polyacrylamide coated capillary electrophoresis column was established. In this method, the untreated capillary column is directly treated with a solution containing a certain proportion of acrylamide, a crosslinking agent, methylidenebisacrylamide, a silylating agent vinyltriethoxysilane, and an initiator azobis Butyryl acetone solution for dynamic coating, and then heat treatment to the monomer crosslinking and polymer in the capillary wall of the bonding reaction occurs at the same time. The prepared column can effectively inhibit the electroosmotic flow and protein adsorption on the capillary wall. When the basic protein was separated in a buffer of pH 4.0, the average column efficiency was 9.1 × 10 5 theoretical plates / meter. The separation efficiency of acidic protein in pH 8.5 buffer was 2.0 × 10 4 theoretical plates / meter. The acid-base protein has good reproducibility of migration time, and the prepared column shows better stability in the pH range of 2.5-8.5. The method also has a higher success rate of column making